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Fargaria ananassa, Prunus armeniaca, Prunus persica, polyphenols, flavonoids, tannin, antioxidant activity.
Purpose: Fruits are important source of phytochemicals and antioxidants such as polyphenols, flavonoїds, tannins, cartenoides and vitamins. The aim of this study is the screening of polyphenols and the evaluation of antioxidant activity in vitro using different methods and in vivo of Fargaria ananassa, Prunus armeniaca and Prunus persica (Rosacea family) fruits extracts.
Methods: Polyphenols in the extracts of fruits was identified by UPLC-DAD. The antioxidant activity of extracts were estimated by methods of the scavenging activity against DPPH, ABTS, hydroxyl radicals (HO.), β-carotene/linoleic acid model system, lipid peroxidation, reducing power and chelating activity. Total phenolics and flavonoids content in these extracts were determined using Folin–Ciocalteu’s reagent and Aluminum chloride colorimetric methods respectively. In vivo antioxidant activity was conducted using male wistar rats.
Result: The results showed that Fargaria ananassa contains high amount of polyphenols and flavonoids (310 ± 0.003µg gallic acid /mg extract and 14.78 ± 0.001µg quercetin equivalent/mg extract respectively ), followed by Prunus armeniaca and Prunus persica, but the highest levels of tannin were found in Prunus armeniaca. Fargaria extract have a high antiradical effect towards DPPH radical, however, Prunus persica have a good effect in the inhibition of lipid peroxidation with Fargaria. The administrations of Fargaria extract (200 mg/kg and 600 mg/kg) elevates the plasma antioxidant activity and reduced the level of MDA with 85.07 ± 2.06% and 39.54 ± 1.11% respectively and increased the levels of GSH in the liver of rats. The UPLC analysis of extracts demonstrated the presence of various phenolic acids (gallic acid, cinnamic acid and hydrocinnamic acid) and flavonoids (rutin and flavons3-ols) in all extracts.
Conclusion: These results support the idea that the consumption of fruits can reduce the risk of disease related to free radicals such as cancer, cardiovascular diseases, hypertension, diabetes and stroke. This effect can be attributed at least in part to the antioxidant properties of polyphenols present in the extracts.