The effect of short-term royal jelly supplement on testosterone levels in sedentary and healthy individuals ROYAL JELLY AND TESTOSTERONE

Main Article Content

ALİ MUHİTTİN TAŞDOĞAN
ZARİFE PANCAR
MUSTAFA ÖZDAL
MEHMET VURAL
SERKAN PANCAR
YAKUP ZÜHTÜ BİRİNCİ

Keywords

GENDER HORMONE, ROYAL JELLY, SUPPLEMENT

Abstract

This study with a placebo-controlled experimental design intends to investigate the effect of Royal Jelly (RJ) on the testosterone levels in sedentary men at a dose of 1000 mg/day. For this purpose, a total of 20 adult sedentary men aged 21 to 23 were included in this study. The subjects visited the laboratory every day for 15 days between 08:00 and 10:00 to get their portion of royal jelly. The subjects were randomly divided into two groups, namely the experimental group (n = 10 individuals, 1000 mg/day of Royal Jelly) and the placebo group (n = 10 individuals, corn starch mixed with 1000 mg/day of water) and they took royal jelly in glass vials at the same time. Blood samples were taken from both groups of subjects one day before and one day after the study and analyzed to determine their testosterone levels. 2x2 mixed factor ANOVA and LSD tests were used to analyze data obtained from the experimental and the placebo group. A sharp increase in the testosterone levels of the experimental group that took RJ for a short time was found to be statistically significant (p<0.05). The pre- and post-test values of the placebo group were not found to be statistically significant (p>0.05). The study shows that a 1000 mg/day dose of RJ supplements was effective in increasing testosterone levels in sedentary, healthy men.

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References

Abdelhafiz, A. T., Muhamad, J. A. (2008). Midcycle pericoital intravaginal bee honey and royal jelly for male factor infertility. International Journal of Gynecology & Obstetrics, 101(2), 146-149.
Al-Masrı, A. (2011) The royal jelly. Honeybee kingdom and its derivaton, In Bartolome, J A A L F M P (ed.) Arabic Book House Publishers; pp.291-300.
Al-Mufarrej, S I; El-Sarag, M S A (1997). Effects of royal jelly on the humoral antibody response and bloodchemistry of chickens. Journal of Applied Animal Research 12 (1): 41-47.
Arzı, A; Olapour, S; Yaghootı, H; Karampour, N (2015). Effect of Royal Jelly on Formalin Induced-Inflammation in Rat Hind Paw. Jundishapur J Nat Pharm Prod 10(1): e22466.
Batchelder, T. (2002). A novel mechanism of liver enhancement from a Traditional bee product. (Medical Anthropology). Townsend Letter for Doctors and Patients, (233), 46-49.
Bincoletto, C., Eberlin, S., Figueiredo, C. A., Luengo, M. B., and Queiroz, M. L. (2005). Effects produced by Royal Jelly on haematopoiesis: relation with host resistance against Ehrlich ascites tumour challenge. International immunopharmacology, 5(4), 679-688.
Biochemical and Biophysical Research Communications 282 (4): 865-874.
Bogdanov, S. (2011). Royal jelly, bee brood: composition, health, medicine: a review. Lipids, 3(8), 8-19.
Bonomı, A (2001) La gelatina reale nell'alimentazione dei suini in fase di svezzamento. Rivista di Suinicoltura 42 (4): 183-188.
Bonomı, A; Bonomı, B M; Quarantellı, A (2001) Royal jelly in turkey feeding; Royal jelly in guinea-fowl feding. Rivista di Scienza dell'Alimentazione 30 (1): 49-60.
Boselli, E., Caboni, M. F., Sabatini, A. G., Marcazzan, G. L., and Lercker, G. (2003). Determination and changes of free amino acids in royal jelly during storage. Apidologie, 34(2), 129-137.
Boukraâ, L., Niar, A., Benbarek, H., and Benhanifia, M. (2008). Additive action of royal jelly and honey against Staphylococcus aureus. Journal of medicinal food, 11(1), 190-192.
Chauvın, R (1968) Action physiologique et therapeutique des produits de la ruche Traite de biologie de l'abeille,Masson; Paris; pp 116-154.
Chauvın, R (1987) La ruche et l'homme. Calmann-Lévy, France.
Cho, Y T (1977) Studies on royal jelly and abnormal cholesterol and triglycerides. American Bee Journal 117: 36-38.
Dawson, H; Rubottom, R; Harrıs, L (1962) The effect of administration of royal jelly on the differentiation and growth of newborn rats. The Anatomical record 142: 123-129.
Delkhoshe-Kasmaıe, F; Malekınejad, H; Khoramjouy, M; Rezaeı-Golmısheh, A; Janbazeacyabar,H (2014) Royal jelly protects from taxol-induced testicular damages via improvement ofantioxidant status and up-regulation of E2f1. Systems Biology in Reproductive Medicine 60 (2): 80-88.
El-Hanoun, A M; Elkomy, A E; Fares, W A; Shahıen, E (2014) mpact of royal jelly to improve reproductive performance of male rabbits under hot summer conditions. World Rabbit Science 22: 241-248.
Elnagar, SA. (2010). Royal jelly counteracts bucks’“summer infertility”. Animal reproduction science, 121(1-2), 174-180.
Eshtiyaghi, M., Deldar, H., Pirsaraei, Z. A., and Shohreh, B. (2016). Royal jelly may improve the metabolism of glucose and redox state of ovine oocytes matured in vitro and embryonic development following in vitro fertilization. Theriogenology, 86(9). 2210-2221.
Fujıı, A., Kobayashı, S., Kuboyama, N., Furukawa, Y., Kaneko, Y., Ishıhama, S., and Tamura, T. (1990). Augmentation of wound healing by royal jelly (RJ) in streptozotocin-diabetic rats. The Japanese Journal of Pharmacology, 53(3), 331-337.
Gasic, S., Vucevic, D., Vasilijic, S., Antunovic, M., Chinou, I., and Colic, M. (2007). Evaluation of the immunomodulatory activities of royal jelly components in vitro. Immunopharmacology and Immunotoxicology, 29(3-4), 521-536.
Gıordano, A; Trenta, A; Mazza, L (1959) [Research on the eventual radioprotective action of royal jelly on the mouse. Experimental contribution]. Radiobiologia, Radioterapia e Fisica Medica 14: 423-439.
Gonnard P, N'guyen CC (1957). Action of royal jelly on oxygen consumption in tissues in vitro. Annales pharmaceutiques francaises. 15(6): 383-393.
Guo, H., Ekusa, A., Iwai, K., Yonekura, M., Takahata, Y., and Morimatsu, F. (2008). Royal jelly peptides inhibit lipid peroxidation in vitro and in vivo. Journal of nutritional science and vitaminology, 54(3), 191-195.
Guo, H., Kouzuma, Y., and Yonekura, M. (2009). Structures and properties of antioxidative peptides derived from royal jelly protein. Food Chemistry, 113(1), 238-245.
Hashimoto, M., Kanda, M., Ikeno, K., Hayashi, Y., Nakamura, T., Ogawa, Y., and Furukawa, S. (2005). Oral administration of royal jelly facilitates mRNA expression of glial cell line-derived neurotrophic factor and neurofilament H in the hippocampus of . the adult mouse brain. Bioscience, Biotechnology, and Biochemistry, 69(4), 800805.
Hassan, A (2009) Effect of royal jelly on sexual efficiency in adult male rats. Iraq J.Vet.Sci. 23: 155-160.
Hattori, N., Ohta, S., Sakamoto, T., Mishima, S., and Furukawa, S. (2011). Royal jelly facilitates restoration of the cognitive ability in trimethyltin-intoxicated mice. Evidence-based complementary and alternative medicine.
Herbert, E. W., Graham, J. M. (1992). The hive and the honey bee. 197-233.
Hınglaıs, H; Hınglaıs, M; Gautherıe, J (1956) [Hormonal study of royal jelly; research on the gonadotrophic principles and substances of estrogenic action]. Comptes rendus des Séances del'Academiedes Sciences 242 (20): 2482-2483.
Honda, Y; Fujıta, Y; Maruyama, H; Arakı, Y; Ichıhara, K; Sato, A; Kojıma, T; Tanaka, M; Nozawa, Y; Ito, M; Honda, S (2011) Lifespan-Extending Effects of Royal Jelly and Its Related Substances on the Nematode Caenorhabditis elegans. Plos One 6 (8).
Howe, S. R., Dimick, P. S., and Benton, A. W. (1985). Composition of freshly harvested and commercial royal jelly. Journal of Apicultural Research, 24(1), 52-61.
Huseın, M Q; Haddad, S G (2006) A new approach to enhance reproductive performance in sheep using royal jelly in comparison with equine chorionic gonadotropin. Animal Reproduction Science 93 (1-2): 24-33.
Huseın, M Q; Krıdlı, R T (2002) Reproductive responses following royal jelly treatment administered orally or intramuscularly into progesterone-treated Awassi ewes. Animal Reproduction Science 74 (1-2): 45-53.
Inoue, S. I., Koya-Miyata, S., Ushio, S., Iwaki, K., Ikeda, M., and Kurimoto, M. (2003). Royal Jelly prolongs the life span of C3H/HeJ mice: correlation with reduced DNA damage. Experimental gerontology, 38(9), 965-969.
Jamnik, P., Goranovič, D., and Raspor, P. (2007). Antioxidative action of royal jelly in the yeast cell. Experimental gerontology, 42(7), 594-600.
Kamakura, M (2002) Signal transduction mechanism leading to enhanced proliferation of primary cultured adult rat hepatocytes treated with royal jelly 57kDa protein. Journal of Biochemistry 132 (6): 911-919.
Kamakura, M; Mıtanı, N; Fukuda, T; Fukushıma, M (2001) Antifatigue effect of fresh royal jelly in mice. Journal Of Nutrıtıonal Scıence And Vıtamınology 47 (6): 394-401.
Kamakura, M; Suenobu, N; Fukushıma, M (2001) Fifty-seven-kDa protein in royal jelly enhances proliferation of primary cultured rat hepatocytes and increases albumin production in the absence of serum.
Kohguchı, M; Inoue, S; Ushıo, S; Iwakı, K; Ikeda, M; Kurımoto, M (2004) Effect of royal jelly diet on the testicular function of hamsters. Food Science and Technology Research 10 (4): 420-423.
Kohno, K; Okamoto, I; Sano, O; Araı, N; Iwakı, K; Ikeda, M; Kurımoto, M (2004) Royal Jelly inhibits the production of proinflammatory cytokines by activated macrophages. Bioscience Biotechnology and Biochemistry 68 (1): 138-145.
Koya-Miyata, S., Okamoto, I., Ushio, S., Iwaki, K., Ikeda, M., and Kurimoto, M. (2004). Identification of a collagen production-promoting factor from an extract of royal jelly and its possible mechanism. Bioscience, Biotechnology, and Biochemistry, 68(4), 767-773.
Krıdlı, R T; Al Khetıb, S S (2006) Reproductive responses in ewes treated with eCG or increasing doses of royal jelly. Animal Reproduction Science 92 (1-2): 75-85.
Krıdlı, R T; Huseın, M Q; Humphrey, W D (2003) Effect of royal jelly and GnRH on the estrus synchronization and pregnancy rate in ewes using intravaginal sponges. Small Ruminant Research 49 (1):25-30.
Krylov, V; Sokolskıı C. (2000) Royal jelly (in Russian). Agroprompoligrafist Krasnodar; pp:214.
Kubo, T., Sasaki, M., Nakamura, J., Sasagawa, H., Ohashi, K., Takeuchi, H., and Natori, S. (1996). Change in the expression of hypopharyngeal-gland proteins of the worker honeybees (Apis mellifera L.) with age and/or role. The Journal of Biochemistry,119(2). 291-295.
Kurkure, N V; Kognole, S M; Pawar, S P; Ganorkar, A G; Bhandarkar, A G; Ingle, V C; Kalorey, D R (2000) Effect of royal jelly as immunonomodulator in chicks. Journal of Immunology & Immunopathology 2 (1/2): 84-87.
Kuzına, N G (1987). Action of apilak on the electrolyte and catecholamine content of the wall of arterial vessels at different levels of systemic arterial pressure. Farmakologiia i Toksikologiia 50 (3): 46-49.
Lercker G, Capella P, Conte LS, Ruini F. (1981). Components of royal jelly: I. Identification of the organic acids. Lipids, 16(12), 912-9.
Lewis, R. (2008). The infertility cure: The ancient Chinese wellness program for getting pregnant and having healthy babies. Little, Brown.
Mannoor, M K; Shımabukuro, I; Tsukamotoa, M; Watanabe, H; Yamaguchı, K (2009) Honeybee royal jelly inhibits autoimmunity in SLE-prone NZB x NZW F1 mice. Lupus (1): 44-52.
Mannoor, M K; Tsukamoto, M; Watanabe, H; Yamaguchı, K; Sato, Y (2008) The Efficacy Of Royal jelly in the restoration of stress-induced disturbance of lymphocytes and granulocytes. Biomedical Research-India 19 (2): 69-77.
Matsui, T., Yukiyoshi, A., Doi, S., Sugimoto, H., Yamada, H., and Matsumoto, K. (2002). Gastrointestinal enzyme production of bioactive peptides from royal jelly protein and their antihypertensive ability in SHR. The Journal of nutritional biochemistry, 13. 80-86.
Mıshıma, S; Suzukı, K M; Isohama, Y; Kuratsu, N; Arakı, Y; Inoue, M; Mıyata, T (2005) Royal jelly has estrogenic effects in vitro and in vivo. Journal of Ethnopharmacology 101 (1-3): 215-220.
Nakaya, M., Onda, H., Sasaki, K., Yukiyoshi, A., Tachibana, H., and Yamada, K. (2007). Effect of royal jelly on bisphenol A-induced proliferation of human breast cancer cells. Bioscience, Biotechnology, and Biochemistry, 71(1), 253-255.
O'Connor, K. J., Baxter, D. (1985). The demonstration of insulin-like material in the honey bee, Apis mellifera. Comparative Biochemistry and Physiology Part B: Comparative Biochemistry, 81(3), 755-760.
Oka, H; Emorı, Y; Kobayashı, N; Hayashı, Y; Nomoto, K (2001) Suppression of allergic reactions by royal jelly in association with the restoration of macrophage function and the improvement of Th1/Th2 cell responses. International immunopharmacology 1 (3): 521-532.
Okamoto, I., Taniguchi, Y., Kunikata, T., Kohno, K., Iwaki, K., Ikeda, M., and Kurimoto, M. (2003). Major royal jelly protein 3 modulates immune responses in vitro and in vivo. Life Sciences, 73(16), 2029-2045.
Park, H M; Cho, M H; Cho, Y; Kım, S Y (2012) Royal Jelly Increases Collagen Production in Rat Skin After Ovariectomy. Journal of Medicinal Food 15 (6): 568-575.
Park, H M; Hwang, E; Lee, K G; Han, S M; Cho, Y; Kım, S Y (2011) Royal Jelly Protects Against Ultraviolet B-Induced Photoaging in Human Skin Fibroblasts via Enhancing Collagen Production. Journal of Medicinal Food 14 (9): 899-906.
Pejcev, P; Bojadzıev, S; Marovskı, T (1965) [The Influence Of Royal Jelly On The Course Of Radıatıon Sıckness In Whıte Rats]. Folia Med.(Plovdiv.) 48: 69-73.
Pejtscheff, P; Belewa-Stajkowa, P; Atanassoff, N (1975) Einfluss des Weiselfuttersaftes auf den Sauerstoffverbrauch und die Aktivität der Adenosintriphosphatase in den Geweben weisser Mäuse Der XXV. Internationale Bienenzüchterkongress Grenoble - Frankreich 1975, Apimondia-Verlag; Bukarest, Rumänien; pp 248-251.
Pyrzanowska, J; Pıechal, A; Blecharz-Klın, K; Graıkou, K; Wıdy-Tyszkıewıcz, E; Chınou, I (2012) Chemical analysis of Greek royal jelly - Its influence of the long-term administration on spatial memory in aged rats. Planta medica 78 (11): 1248.
Ragab, S S; Ibrahım, M K (1999) Evaluation of some chemical, antibacterial and biological properties of fresh and refrigerated royal jelly. Egyptian Journal of Microbiology 34 (1): 115-128.
Sauerwald, N., Polster, J., Bengsch, E., Niessen, L., and Vogel, R. F. (1998). Combined antibacterial and antifungal properties of water soluble fractions of royal jelly. Advances in Food Sciences, 20, 46-52.
Savouret, J.F., Quesne, M. (2002). Resveratrol and cancer. Biomed Pharmacother, 56, 84-87.
Shahzada, Q; Mehmoodb, M; Khanc, H; Husnad, A; Qadeerd, S; Azamd, A; Naseere, Z;Ahmadf, E; Safdarg, M; Ahmadb, M (2016) Royal jelly supplementation in semen extender enhances post-thaw quality and fertility of Nili-Ravi buffalo bull sperm. Animal Reproduction Science 167: 83-88.
Shen, X; Lu, R; He, G (1995) Effects of lyophilized royal jelly on experimental hyperlipaemia and thrombosis 1434. Zhonghua Yufang Yixue Zazhi 29 (1): 27-29.
Simuth, J., & Bilikova, K. (2004). Potential contribution of royal jelly proteins for health. Honeybee Science-Tamagawa University (Japan).
Stocker, A., Schramel, P., Kettrup, A., and Bengsch, E. (2005). Trace and mineral elements in royal jelly and homeostatic effects. Journal of Trace Elements in Medicine and Biology, 19(2-3), 183-189.
Suemaru, K., Cui, R., Li, B., Watanabe, S., Okihara, K., Hashimoto, K., and Araki, H. (2008). Topical application of royal jelly has a healing effect for 5-fluorouracilinduced experimental oral mucositis in hamsters. Methods Find Experimental Clinical Pharmacology, 30(2), 103-106.
Suzukı, K M; Isohama, Y; Maruyama, H; Yamada, Y; Narıta, Y; Ohta, S; Arakı, Y; Mıyata, T;Mıshıma, S (2008) Estrogenic activities of fatty acids and a sterol isolated from royal jelly. Evidence-based complementary and alternative medicine 5 (3): 295-302.
Takenaka, T., Yatsunami, K., Echigo, T. (1986). Changes in quality of royal jelly during storage. Nippon Shokuhin Kogyo Gakkaishi, 33(1), 1-7.
Tokunaga, K. H., Yoshida, C., Suzuki, K. M., Maruyama, H., Futamura, Y., Araki, Y., and Mishima, S. (2004). Antihypertensive effect of peptides from royal jelly in spontaneously hypertensive rats. Biological and Pharmaceutical Bulletin, 27(2), 189192.
Vittek, J. (1995). Effect of royal jelly on serum lipids in experimental animals and humans with atherosclerosis. Experientia, 51(9-10), 927-935.
Viuda-Martos, M., Ruiz-Navajas, Y., Fernández López, J., and Pérez Álvarez, J. A. (2008). Functional properties of honey, propolis, and royal jelly. Journal of food science, 73(9).
Vucevıc, D; Mellıou, E; Vasılıjıc, S; Gasıc, S; Ivanovskı, P; Chınou, I; Colıc, M (2007) Fatty acids isolated from royal jelly modulate dendritic cell-mediated immune response in vitro. International immunopharmacology 7 (9): 1211-1220.
Wagner, H; Dobler, I; Thıem, I (1970) [Effect of food-juice of the queen bee (royal jelly) on the peripheral blood and the survival rate of mice after whole body x-irradiation]. Radiobiol.Radiother.(Berl) 11 (3): 323-328.
Watanabe, K; Shınmoto, H; Koborı, M; Tsushıda, T; Shınohara, K; Kanaeda, J; Yonekura, M (1996) Growth stimulation with honey royal jelly DIII protein of human lymphocytic cell lines in a serum-free medium. Biotechnology Techniques 10 (12): 959-962.
Yamada, K; Ikeda, I; Sugahara, T; Shırahata, S; Murakamı, H (1989) Screening of immunoglobulin production stimulating factor (IPSF) in foodstuffs using human-human hybridoma HB4C5 cells 784. Agricultural and Biological Chemistry 53 (11): 2987-2991.

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