Polydioxanone powder induces collagen synthesis in fibroblast culture? – A pilot study

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Ermino Souza
José Ricardo de Albergaria Barbosa https://orcid.org/0000-0001-5127-8318
Claudia Herrera Tambeli https://orcid.org/0000-0001-5566-710X
Carlos Amilcar Parada https://orcid.org/0000-0001-5209-8853
Giancarlo de La Torre Canales https://orcid.org/0000-0002-0921-342X
Mariana Barbosa Câmara de Souza https://orcid.org/0000-0002-9961-121X
Ricardo Cesar Gobbi de Oliveira https://orcid.org/0000-0002-0725-2337
Celia Marisa Rizzatti-Barbosa https://orcid.org/0000-0002-8747-0034


Collagen. Aging. Fibroblasts. PLLA. Polydioxanone.


Background: Polydioxanone powder appears in the scenario of biostimulators as an effective and minimally invasive alternative to delay the effects of aging on the skin caused by the degradation of collagen and elastic fibers. Objectives: To verify, in vitro, the ability of a polydioxanone powder to induce collagen production in a culture of human fibroblasts and compare the results with the induction promoted by the PLLA powder.

Methods: The study evaluated CCD-1072Sk human fibroblasts in adherent culture (ATCC CRL-2088), into eight groups (n=12) C, PLLA, PDO, PDO # organized into two subgroups PLATES A (24h) AND PLATES B (48h) if Poly-L-lactic acid-PLLA (Rennova ® elleva) and powdered polydioxanone -PDO (UltraCol – Ultra-V ® Medical) would stimulate an increase in collagen production by fibroblasts. The Shapiro-Wilk test was used to analyze data normality. To analyze the effect of the time in which the samples were analyzed (24h or 48h) and of the C, PLLA, PDO, PDO # group on collagen concentrations, we used the Two - Way ANOVA test. To identify significant differences between subgroups, we used Tukey 's post-hoc. Results: Variables are presented as mean and standard deviation (SD). The results showed a significant increase in collagen production from 24h to 48h in the four analyzed groups C, PLLA, PDO and PDO #. Conclusion: None of the biostimulators positively influenced collagen production.

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