Polydioxanone powder induces collagen synthesis in fibroblast culture? – A pilot study
Main Article Content
Keywords
Collagen. Aging. Fibroblasts. PLLA. Polydioxanone.
Abstract
Background: Polydioxanone powder appears in the scenario of biostimulators as an effective and minimally invasive alternative to delay the effects of aging on the skin caused by the degradation of collagen and elastic fibers. Objectives: To verify, in vitro, the ability of a polydioxanone powder to induce collagen production in a culture of human fibroblasts and compare the results with the induction promoted by the PLLA powder.
Methods: The study evaluated CCD-1072Sk human fibroblasts in adherent culture (ATCC CRL-2088), into eight groups (n=12) C, PLLA, PDO, PDO # organized into two subgroups PLATES A (24h) AND PLATES B (48h) if Poly-L-lactic acid-PLLA (Rennova ® elleva) and powdered polydioxanone -PDO (UltraCol – Ultra-V ® Medical) would stimulate an increase in collagen production by fibroblasts. The Shapiro-Wilk test was used to analyze data normality. To analyze the effect of the time in which the samples were analyzed (24h or 48h) and of the C, PLLA, PDO, PDO # group on collagen concentrations, we used the Two - Way ANOVA test. To identify significant differences between subgroups, we used Tukey 's post-hoc. Results: Variables are presented as mean and standard deviation (SD). The results showed a significant increase in collagen production from 24h to 48h in the four analyzed groups C, PLLA, PDO and PDO #. Conclusion: None of the biostimulators positively influenced collagen production.
References
2. Ramos-e-Silva M, da Silva Carneiro SC. Elderly skin and its rejuvenation : products and procedures for the aging skin . J Cosmet Dermatol . 2007;6(1):40-50. doi:10.1111/j.1473-2165.2007.00289.x
3. Chaudhary M, Khan A, Gupta M. Skin Aging : Pathophysiology and Current Market Treatment Approaches. Curr Aging Sci . 2020;13(1):22-30. doi:10.2174/1567205016666190809161115
4. Moon H, Fundaro SP, Goh CL, Hau KC, Paz-Lao P, Salti G. A Review on the Combined Use of Soft Tissue Filler , Suspension Threads, and botulinum Toxin for Facial Rejuvenation . J Cutan aesthet Surg . 2021;14(2):147-155. doi:10.4103/JCAS.JCAS_119_20
5. de Melo F, Nicolau P, Piovano L, et al. Recommendations for volume augmentation and rejuvenation of the face and hands with the new generation polycaprolactone-based collagen stimulator ( Ellansé ® ). clinic cosmetic Investig Dermatol . 2017;10:431-440. Published 2017 Nov 8. doi:10.2147/CCID.S145195
6. de Almeida AT, Figueredo V, da Cunha ALG, et al. Consensus Recommendations for the Use of hyperdiluted Calcium Hydroxyapatite ( Radiesse ) as a Face and Body Biostimulatory Agent. plast rebuild Surg GlobOpen . _ 2019;7(3):e2160. Published 2019 Mar 14. doi:10.1097/GOX.0000000000002160
7. Kim JS. Changes in Dermal Thickness in Biopsy study of histologic Findings After a Single Injection of Polycaprolactone-Based filler into the Dermis . aesthet Surg J. _ 2019;39(12):NP484-NP494. doi:10.1093/ asj /sjz050
8. Christen MO, Vercesi F. Polycaprolactone : How a Well-Known and Futuristic Polymer Has become an Innovative Collagen-Stimulator in Esthetics . clinic cosmetic Investig Dermatol . 2020;13:31-48. Published 2020 Jan 20. doi:10.2147/CCID.S229054
9. de Melo F, Carrijo A, Hong K, et al. minimally invasive aesthetic Treatment of the face and Neck Using Combinations of a PCL- Based collagen Stimulator , PLLA/PLGA Suspension Sutures, and Cross - Linked hyaluronic Acid . clinic cosmetic Investig Dermatol . 2020;13:333-344. Published 2020 May 5. doi:10.2147/CCID.S248280
10. Lin JY, Lin CY. Adjusting Thickness before Injection : A New Trend for Preparing collagen-stimulating Fillers . plast rebuild Surg GlobOpen . _ 2021;9(6):e3653. Published 2021 Jun 24. doi:10.1097/GOX.0000000000003653
11. Martins JA, Lach AA, Morris HL, Carr AJ, Mouthuy PA. polydioxanone implants : A systematic review on safety and performance in patients . J Biomater app . 2020;34(7):902-916. doi:10.1177/0885328219888841
12. Ha YI, Kim JH, Park ES. histological and molecular biological analysis on the reaction of absorbable thread; polydioxanone and polycaprolactone in rat model. J Cosmet Dermatol . 2022;21(7):2774-2782. doi:10.1111/jocd.14587
13. Kwon TR, Han SW, Yeo IK, et al. Biostimulatory effects of polydioxanone , poly - d, lactic acid , and polycaprolactone fillers in mouse model. J Cosmet Dermatol . 2019;18(4):1002-1008. doi:10.1111/jocd.12950
14. Couderot-Masuyer C, et al." Evaluation of the behavior of Wrinkles fibroblasts and Normal Aged Fibroblasts in the Presence of Poly-L- Lactic Acid ." Journal of Cosmetics , Dermatological Sciences and Applications . 2012;2(1):20-27. doi : 10.4236/jcdsa.2012.21006 .
15. AMUSO, D et al. Histological evaluation of a biorevitalization treatment with PDO wires. Aesthetic Medicine.2015;1(3)111-117, 2015. https://ita.calameo.com/read/0043334673ff11b58fa0d
16. Kim CM, Kim BY, Hye Suh D, Lee SJ, Moon HR, Ryu HJ. The efficacy of powdered polydioxanone in terms of collagen production compared with poly -L- lactic acid in a murine model. J Cosmet Dermatol . 2019;18(6):1893-1898. doi:10.1111/jocd.12894
17. Kim SA, Kim HS, Jung JW, Suh SI, Ryoo YW. Poly-L- Lactic acid Increases Collagen Gene Expression and Synthesis in Cultured Dermal Fibroblast (Hs68) Through the p38 MAPK Pathway . Ann Dermatol . 2019;31(1):97-100. doi:10.5021/ad.2019.31.1.97
18. Ray S, Ta HT. Investigating the effect of Biomaterials Such as Poly-(l- Lactic Acid ) Particles on collagen Synthesis In Vitro: Method is Matter . j funct Biomater . 2020;11(3):51. Published 2020 Jul 24. doi:10.3390/jfb11030051
19. COHEN, J. (1988). Statistical Power Analysis for the Behavioral Sciences. New York, NY: Routledge Academic.