Do immunohistochemical studies have a role in predicting prognosis of laryngeal squamous cell carcinomas? CD44 and Fascin experience

Do immunohistochemical studies have a role in predicting prognosis of laryngeal squamous cell carcinomas? CD44 and Fascin experience

Authors

  • Çiğdem Öztürk Recep Tayyip Erdogan University Training and Research Hospital, Pathology Department, Rize, Merkez, Rize, Turkey
  • Hüsniye Esra Paşaoğlu University of Health Science Bagcilar Training and Research Hospital, Pathology Department
  • Funda Emre University of Health Science Bagcilar Training and Research Hospital, Pathology Department
  • Ümit Seza Tetikkurt Demiroglu Bilim University Medical Faculty, Pathology Department
  • Tülin Şentürk Ege University of Health Science Bagcilar Training and Research Hospital, Otolaryngology Department

Keywords:

Larynx, squamous cell carcinoma, CD44, Fascin

Abstract

Background and objectives: The diagnosis of laryngeal squamous cell carcinoma (LSCC) can be made easily based on histopathological findings, but the relationship between morphological findings and prognosis is not clear. In addition to morphological findings, the use of novel markers may contribute to the development of new treatment strategies and improved patient prognosis. CD44, which is a cancer stem cell marker, and Fascin-1, an actin-binding protein has been associated with poor prognosis in many tumors. The aim of this study was to investigate the relationship between CD44 and Fascin-1 expression and clinicopathologic parameters in LSCC and their roles in the determination of clinical behavior and prognosis. The aim of this study is to investigate whether CD44 and Fascin have a relationship with clinicopathological parameters and have a role in determining clinical behavior and prognosis in LSCC.

Methods: 130 patients who were operated in our hospital for LSCC between 2012 and 2018 were included in this study. Fascin-1 and CD44 stains were applied immunohistochemically to the paraffin blocks of the tumors. Immunostained specimens were scored according to the intensity of staining and the percentage of staining for each marker. Overall scores were summed and was designated as immunoreactivity score (IRS). Finally, IRS was categorized into two groups; Low and High CD44/Fascin IRS.

Results: There were no statistically significant differences between low and high CD44 and Fascin IRS groups in terms of clinicopathologic parameters, overall and disease-free survival (p> 0.05).

Conclusion: Immunhistochemical studies are not yet sufficient to predict patient prognosis. Morphological findings still remain of priority and importance for pathologists.

References

Cattaruzza MS, Maisonneuve P, Boyle P. Epidemiology of laryngeal cancer. Eur J Cancer B Oral Oncol. 1996;32B(5):293-305.

Stelow BE. The Larynx, Sternberg’s Diagnostic Surgical Pathology, 6th editio. Stacey ME, New York, Lippincott Williams and Wilkins 2015: 903-928.

Huang Q, Yu GP, McCormick SA, et al. Genetic differences detected by comparative genomic hybridization in head and neck squamous cell carcinomas from different tumor sites: construction of oncogenetic trees for tumor progression. Genes Chromosomes Cancer. 2002;34(2):224-233..

Trapasso S, Allegra E. Role of CD44 as a marker of cancer stem cells in head and neck cancer. Biologics. 2012;6:379-383.

Sun S, Pan X, Zhao L, Zhou J, Wang H, Sun Y. The Expression and Relationship of CD68-Tumor-Associated Macrophages and Microvascular Density With the Prognosis of Patients With Laryngeal Squamous Cell Carcinoma. Clin Exp Otorhinolaryngol. 2016;9(3):270-277.

Kokko LL, Hurme S, Maula SM, et al. Significance of site-specific prognosis of cancer stem cell marker CD44 in head and neck squamous-cell carcinoma. Oral Oncol. 2011;47(6):510-516.

Esteban F, Bravo JJ, González-Moles MA, Bravo M, Ruiz-Avila I, Gil-Montoya JA. Adhesion molecule CD44 as a prognostic factor in laryngeal cancer. Anticancer Res. 2005;25(2A):1115-1121.

Yan Y, Zuo X, Wei D. Concise Review: Emerging Role of CD44 in Cancer Stem Cells: A Promising Biomarker and Therapeutic Target. Stem Cells Transl Med. 2015;4(9):1033-1043.

Du ZP, Wu BL, Xie JJ, et al. Network Analyses of Gene Expression following Fascin Knockdown in Esophageal Squamous Cell Carcinoma Cells. Asian Pac J Cancer Prev. 2015;16(13):5445-5451.

Gao W, Zhang C, Feng Y, et al. Fascin-1, ezrin and paxillin contribute to the malignant progression and are predictors of clinical prognosis in laryngeal squamous cell carcinoma. PLoS One. 2012;7(11):e50710.

Slootweg GJR. Tumours of the hypopharynx, larynx, trachea and parapharyngeal space. Who Classification of Head and Neck. 4th editio., SP Naggar AK, Chan J, Grandis J, Takata T, Lyon, IARC Press, 2017; 8–104.

Broders AC. Squamous cell epithelioma of the lip. J Am Med Assoc. 1920;74:656–664.

Bryne M, Jenssen N, Boysen M. Histological grading in the deep invasive front of T1 and T2 glottic squamous cell carcinomas has high prognostic value. Virchows Arch. 1995;427(3):277-281.

Wiernik G, Millard PR, Haybittle JL. The predictive value of histological classification into degrees of differentiation of squamous cell carcinoma of the larynx and hypopharynx compared with the survival of patients. Histopathology. 1991;19(5):411-417.

Almangush A, Bello IO, Keski-Säntti H, et al. Depth of invasion, tumor budding, and worst pattern of invasion: prognostic indicators in early-stage oral tongue cancer. Head Neck. 2014;36(6):811-818.

Perez-Ordoñez B, Beauchemin M, Jordan RC. Molecular biology of squamous cell carcinoma of the head and neck. J Clin Pathol. 2006;59(5):445-453.

Zöller M. CD44: can a cancer-initiating cell profit from an abundantly expressed molecule?. Nat Rev Cancer. 2011;11(4):254-267.

Bartakova A, Michalova K, Presl J, Vlasak P, Kostun J, Bouda J. CD44 as a cancer stem cell marker and its prognostic value in patients with ovarian carcinoma. J Obstet Gynaecol. 2018;38(1):110-114.

Nam KH, Yoon H, Lee K, et al. Predictive value for lymph node metastasis of epithelial-mesenchymal transition and cancer stem cell marker expression in early gastric cancer. Pathol Res Pract. 2017;213(9):1221-1226.

Krump M, Ehrmann J. Differences in CD44s expression in HNSCC tumours of different areas within the oral cavity. Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2013;157(4):280-283.

Boxberg M, Götz C, Haidari S, et al. Immunohistochemical expression of CD44 in oral squamous cell carcinoma in relation to histomorphological parameters and clinicopathological factors. Histopathology. 2018;73(4):559-572.

Güler G, Saraç S, Uner A, Karabulut E, Ayhan A, Hiroshi O. Prognostic value of CD44 variant 6 in laryngeal epidermoid carcinomas. Arch Otolaryngol Head Neck Surg. 2002;128(4):393-397.

de Jong MC, Pramana J, van der Wal JE, et al. CD44 expression predicts local recurrence after radiotherapy in larynx cancer. Clin Cancer Res. 2010;16(21):5329-5338.

Cao HH, Zhang SY, Shen JH, et al. A three-protein signature and clinical outcome in esophageal squamous cell carcinoma. Oncotarget. 2015;6(7):5435-5448.

Kureishy N, Sapountzi V, Prag S, Anilkumar N, Adams JC. Fascins, and their roles in cell structure and function. Bioessays. 2002;24(4):350-361.

Hashimoto Y, Skacel M, Adams JC. Roles of fascin in human carcinoma motility and signaling: prospects for a novel biomarker?. Int J Biochem Cell Biol. 2005;37(9):1787-1804.

Jawhari AU, Buda A, Jenkins M, et al. Fascin, an actin-bundling protein, modulates colonic epithelial cell invasiveness and differentiation in vitro. Am J Pathol. 2003;162(1):69-80.

Roseweir AK, Kong CY, Park JH, et al. A novel tumor-based epithelial-to-mesenchymal transition score that associates with prognosis and metastasis in patients with Stage II/III colorectal cancer. Int J Cancer. 2019;144(1):150-159.

Zhang H, Xu L, Xiao D, et al. Fascin is a potential biomarker for early-stage oesophageal squamous cell carcinoma. J Clin Pathol. 2006;59(9):958-964.

Chen WX, Hong XB, Hong CQ, et al. Tumor-associated autoantibodies against Fascin as a novel diagnostic biomarker for esophageal squamous cell carcinoma. Clin Res Hepatol Gastroenterol. 2017;41(3):327-332.

Rodrigues PC, Sawazaki-Calone I, Ervolino de Oliveira C, et al. Fascin promotes migration and invasion and is a prognostic marker for oral squamous cell carcinoma. Oncotarget. 2017;8(43):74736-74754.

Durmaz A, Kurt B, Ongoru O, Karahatay S, Gerek M, Yalcin S. Significance of fascin expression in laryngeal squamous cell carcinoma. J Laryngol Otol. 2010;124(2):194-198.

Papaspyrou K, Brochhausen C, Schmidtmann I, et al. Fascin upregulation in primary head and neck squamous cell carcinoma is associated with lymphatic metastasis. Oncol Lett. 2014;7(6):2041-2046.

Zou J, Yang H, Chen F, et al. Prognostic significance of fascin-1 and E-cadherin expression in laryngeal squamous cell carcinoma. Eur J Cancer Prev. 2010;19(1):11-17.

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Published

19-01-2022

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ORIGINAL ARTICLES

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1.
Do immunohistochemical studies have a role in predicting prognosis of laryngeal squamous cell carcinomas? CD44 and Fascin experience. Acta Biomed [Internet]. 2022 Jan. 19 [cited 2024 Mar. 29];92(6):e2021309. Available from: https://www.mattioli1885journals.com/index.php/actabiomedica/article/view/10432