Bottle gourd (Lagenaria siceraria) extracts improve glucose and lipid metabolism in 3T3-L1 adipocytes

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Assia Sassi
Muhammad Muzaffar Ali Khan Khattak
Muhammad Taher


adipocytes, adipogenesis, adipolysis, glucose uptake, adiponectin, leptin


The aim of this study was to investigate the effect of bottle gourd which belongs to family of cucurbitaceae (Lagenaria Siceraria (LS) on hyperglycemia by assessing the cells viability, adipogenesis, adipolysis, glucose uptake, adiponectin and leptin using 3T3-L1 adipocytes as a model. Fresh bottle gourd were washed thoroughly with distilled water and segregated into three parts namely; Fresh bottle gourd were washed thoroughly with distilled water and segregated into three parts namely; whole vegetable (LSW), peels (LSP) and seeds (LSS). Each part was blended either with water or ethanol which then labeled as LSWw, LSPw, LSSw LSWe, LSPe and LSSe, respectively. The collected data was compiled and statistical analysis was performed. Mainly, one-way analysis of variance (ANOVA) was performed followed by Tukey’s post-hoc test to ascertain differences in the means. The lipid droplet formation was significantly (P<0.001) higher in the adipocytes treated with the extracts of LSWw, LSSw, LSWe , LSSe and lower in the extracts of LS peels (LSPw, LSPe) compared to induction medium (MDI)/insulin (control). The same extracts also significantly (P<0.001) increased glycerol release during adipolysis compared to the control. It caused a significant (P<0.001) increase in adiponectin concentrations for LSPw, LSWw and a decrease in leptin concentrations for the water extracts of the LSPw. The present study showed that there was a hypoglycaemic effect of LS extracts by improving the regulation of adipogenesis through the formation of lipid droplets, adipolysis by increasing the release of glycerol, glucose uptake by increasing the uptake into the cells as well as adiponectin and leptin concentrations which could be of clinical importance in energy regulation which is a key factor in diabetes, obesity and metabolic syndrome. 


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