Inhibiting insulin resistance mechanisms by DTS phytocompound: an experimental study on metabolic syndrome-prone adipocytes

Inhibiting insulin resistance mechanisms by DTS phytocompound: an experimental study on metabolic syndrome-prone adipocytes

Authors

  • R. Catanzaro
  • A. Lorenzetti
  • F. Allegri, et al.

Keywords:

Nuclear factor-κB, cytokines, oxidative stress, DTS

Abstract

The present study was designed to determine whether DTS a phytocompound endowed with antioxidant properties, could beneficially modulate nitric oxide (NO) production stimulated by lipopolysaccharide (LPS) and tumor necrosis factor-α (TNF-α) in adipocytes. Combined stimulation (CS-treatment) exerted by using 5 μg/ml of LPS together with 100 ng/ml of TNF-α significantly enhanced NO production in 3T3-L1 adipocytes. Preincubation of the adipocytes with DTS (10-30 mM) inhibited such phenomenon in a dose-dependent fashion. The production of NO was decreased by 52% at the concentration of 30mM of DTS. The decrease in NO production by DTS was associated also with a decrease in inducible nitric oxide synthase (iNOS) protein and iNOS mRNA expression. Nuclear factor-kappa B (NF-κB) was significantly enhanced by CS-treatment, while the pretreatment with 30 mM of DTS prevented the activity by 27%. IL-6 production in 3T3-L1 adipocytes was markedly increased by CS stimulus, and the enhanced secretion of IL-6 was suppressed in a dose-dependent manner by DTS. These results suggest that DTS regulates iNOS expression and NO production in adipocytes through the modulating activation of NF-κB and may have a potential clinical application within protocols designed for treating metabolic syndrome.

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Published

01-10-2012

Issue

Section

ORIGINAL ARTICLES

How to Cite

1.
Catanzaro R, Lorenzetti A, Allegri, et al. F. Inhibiting insulin resistance mechanisms by DTS phytocompound: an experimental study on metabolic syndrome-prone adipocytes. Acta Biomed [Internet]. 2012 Oct. 1 [cited 2024 Jul. 15];83(2):95-102. Available from: https://www.mattioli1885journals.com/index.php/actabiomedica/article/view/2368