Simple and fast orotracheal intubation procedure in rats

Simple and fast orotracheal intubation procedure in rats

Authors

  • Giovanni Tomasello BIONEC DEPARTMENT - SHOOL OF MEDICINE AND SURGERY, PALERMO, ITALY
  • Francesco Damiani
  • Giovanni Cassata
  • Vincenzo Davide Palumbo
  • Emanuele Sinagra
  • Provvidenza Damiani
  • Antonino Bruno
  • Luca Cicero
  • Francesco Cupido
  • Francesco Carini
  • Attilio Ignazio Lo Monte

Keywords:

pserimental clinical procedure,

Abstract

Abstract: Introduction: Endotracheal intubation in the rat is difficult because of the extremely small size of anatomical structures (oral cavity, epiglottis and vocal cords), small inlet for an endotracheal tube and the lack of proper technical instruments. Material and Methods: In this study we used seventy rats weighting 400–500 g. The equipment needed for the intubation was an operating table, a longish of cotton, a cotton tip, orotracheal tube, neonatal laryngoscope blades, KTR4 small animal ventilator and isoflurane for inhalation anaesthesia. Premedication was carried out by medetomidine hydrochloride 1 mg/mL; then, thanks to a closed glass chamber, a mixture of oxygen and isoflurane was administered. By means of a neonatal laryngoscope the orotracheal tube was advanced into the oral cavity until the wire guide was visualized trough the vocal cords; then it was passed through them. The tube was introduced directly into the larynx over the wire guide; successively, the guide was removed and the tube placed into the trachea. Breathing was confirmed using a glove, cut at the end of a finger, simulating a small balloon. Results: We achieved a fast and simple orotracheal intubation in all animals employed. Conclusions: We believe that our procedure is easier and faster than those previously reported in scientific literature.

Author Biography

Giovanni Tomasello, BIONEC DEPARTMENT - SHOOL OF MEDICINE AND SURGERY, PALERMO, ITALY

Professore Aggregato di Chirurgia Generale MED/18

Università degli Studi di Palermo

Facoltà di Medicina e Chirurgia

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Published

06-05-2016

How to Cite

1.
Simple and fast orotracheal intubation procedure in rats. Acta Biomed [Internet]. 2016 May 6 [cited 2024 Jun. 24];87(1):13-5. Available from: https://www.mattioli1885journals.com/index.php/actabiomedica/article/view/4479