The Effect of Probiotic Use on Some Blood Parameters in Overweight and Obese Women Who Follow a Weight-Loss Diet The Probiotic Use on Overweight and Obese Women Following a Weight-Loss Diet

Main Article Content

Yeşim İşgüzar Orak
Sıdıka Bulduk
Mehmet Baştemir

Keywords

weight-loss diet, overweight, obesity, probiotic, body composition

Abstract

Background and aim: This study was conducted to determine the effect of 8-week probiotic use on body composition and blood parameters in overweight and obese women who are on a weight loss diet


Methods: 34 women with a BMI value above 25 kg/m2 participated. The individuals participating in the research were divided into two groups. For eight weeks, the experimental group was given a weight loss diet program, exercise program and oral probiotic nutritional supplement containing various probiotic strains in the amount of 1.5x109 cfu/g in each capsule twice a day; The control group was given a weight loss diet program and an exercise program without probiotic supplementation. Anthropometric measurements of the individuals (height baseline, body weight, waist, hip and neck circumference, body mass index, waist/hip circumference and waist-height ratio) in both groups were evaluated statistically at the initial, 4th and 8th weeks. Some biochemical parameters were measured at the beginning of the study and the end of the 8th week.


Results: A significant difference was found between body weight, body mass index, waist circumference, hip circumference, waist-height ratio, body fat percentage, and body fat mass measurements (p<0,001). Significant improvements were found in LDL-C, fasting insulin, AST and TSH values in both groups (p<0.05). While the HOMA-IR values were significantly lower, HDL-C values were found to be significantly higher at the end of the study in the experimental group (p<0.05).


Conclusions: Balanced hypocaloric diet caused significant improvements in anthropometric measurements and blood parameters in experimental and control groups. The use of probiotics has positive effects on HOMA-IR and HDL-C values in the experimental group.


 

Abstract 260 | PDF Downloads 330

References

1. De Lorenzo A, Gratteri S, Gualtieri P, Cammarano A, Bertucci P, Di Renzo L. Why primary obesity is a disease?. J trans med 2019; 17(1): 169. https://doi.org/10.1186/s12967-019-1919-y.
2. Kim TJ, von dem Knesebeck O. Income and obesity: what is the direction of the relationship? A systematic review and meta-analysis BMJ Open 2018;8:e019862. doi: 10.1136/bmjopen-2017-019862.
3. Kang Y, Cai Y. The development of probiotics therapy to obesity: a therapy that has gained considerable momentum. Hormones (Athens) 2018 Jun;17(2):141-151. doi: 10.1007/s42000-018-0003-y. Epub 2018 Apr 16. PMID: 29858841.
4. NCD Risk Factor Collaboration (NCD-RisC). Trends in adult body-mass index in 200 countries from 1975 to 2014: a pooled analysis of 1698 population-based measurement studies with 19·2 million participants. Lancet. 2016 Apr 2;387(10026):1377-1396. doi: 10.1016/S0140-6736(16)30054-X. Erratum in: Lancet 2016 May 14;387(10032):1998. PMID: 27115820.
5. Nuttall FQ. Body Mass Index: Obesity, BMI, and Health: A Critical Review. Nutrition today 2015; 50(3): 117–128. https://doi.org/10.1097/NT.0000000000000092.
6. Alqunai MS, Alrashid FF. Bariatric surgery for the management of type 2 diabetes mellitus-current trends and challenges: a review article. Am j trans res 2022; 14(2): 1160–1171.
7. Nieuwdorp M, Gilijamse PW, Pai N, Kaplan LM. Role of the microbiome in energy regulation and metabolism. Gastroenterol 2014 May;146(6):1525-33. doi: 10.1053/j.gastro.2014.02.008. Epub 2014 Feb 19. PMID: 24560870.
8. van den Elsen LW, Poyntz HC, Weyrich LS, Young W, Forbes-Blom EE. Embracing the gut microbiota: the new frontier for inflammatory and infectious diseases. Clinical & translational immunology 2017; 6(1), e125. https://doi.org/10.1038/cti.2016.91.
9. Thursby E, Juge N. Introduction to the human gut microbiota. Biochem J 2017 May 16;474(11):1823-1836. doi: 10.1042/BCJ20160510. PMID: 28512250; PMCID: PMC5433529.
10. Olvera-Rosales LB, Cruz-Guerrero AE, Ramírez-Moreno E, et al. Impact of the Gut Microbiota Balance on the Health-Disease Relationship: The Importance of Consuming Probiotics and Prebiotics. Foods (Basel, Switzerland) 2021; 10(6): 1261. https://doi.org/10.3390/foods10061261.
11. Bulduk E, Gönül B, Ozer C. Effects of vitamin C on muscle glycogen and oxidative events in experimental diabetes. Mol Cell Biochem 2006 Nov;292(1-2):131-7. doi: 10.1007/s11010-006-9226-3. Epub 2006 Jun 7. PMID: 16758299.
12. Cani PD, Delzenne NM. The role of the gut microbiota in energy metabolism and metabolic disease. Curr Pharm Des 2009;15(13):1546-58. doi: 10.2174/138161209788168164. PMID: 19442172.
13. Bäckhed F, Ding H, Wang T, et al. The gut microbiota as an environmental factor that regulates fat storage. Proc Natl Acad Sci U S A 2004 Nov 2;101(44):15718-23. doi: 10.1073/pnas.0407076101. Epub 2004 Oct 25. PMID: 15505215; PMCID: PMC524219.
14. Neish AS. Microbes in gastrointestinal health and disease. Gastroenterology. 2009 Jan;136(1):65-80. doi: 10.1053/j.gastro.2008.10.080. Epub 2008; Nov 19. PMID: 19026645; PMCID: PMC2892787.
15. Markowiak P, Śliżewska K. Effects of Probiotics, Prebiotics, and Synbiotics on Human Health. Nutrients 2017 Sep 15;9(9):1021. doi: 10.3390/nu9091021. PMID: 28914794; PMCID: PMC5622781.
16. Tomasello G, Mazzola M, Leone A, et al. Nutrition, oxidative stress and intestinal dysbiosis: Influence of diet on gut microbiota in inflammatory bowel diseases. Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2016 Dec;160(4):461-466. doi: 10.5507/bp.2016.052. Epub 2016 Oct 26. PMID: 27812084.
17. Ley RE, Bäckhed F, Turnbaugh P, Lozupone CA, Knight RD, Gordon JI. Obesity alters gut microbial ecology. Proc Natl Acad Sci U S A. 2005 Aug 2;102(31):11070-5. doi: 10.1073/pnas.0504978102. Epub 2005 Jul 20. PMID: 16033867; PMCID: PMC1176910.
18. Kobyliak N, Conte C, Cammarota G, et al. Probiotics in prevention and treatment of obesity: a critical view. Nutrition & metabolism 2016 Feb 20;13:14. doi: 10.1186/s12986-016-0067-0. PMID: 26900391; PMCID: PMC4761174.
19. Mazlyn MM, Nagarajah LH, Fatimah A, Norimah AK, Goh KL. Effects of a probiotic fermented milk on functional constipation: a randomized, double-blind, placebo-controlled study. J Gastroenterol Hepatol 2013 Jul;28(7):1141-7. doi: 10.1111/jgh.12168. Erratum in: J Gastroenterol Hepatol. 2013 Sep;28(9):1584. PMID: 23432408.
20. Gong J, Bai T, Zhang L, Qian W, Song J, Hou X. Inhibition effect of Bifidobacterium longum, Lactobacillus acidophilus, Streptococcus thermophilus and Enterococcus faecalis and their related products on human colonic smooth muscle in vitro. PLoS One 2017 Dec 7;12(12): e0189257. doi: 10.1371/journal.pone.0189257. PMID: 29216305; PMCID: PMC5720742.
21. Arslan N. Obezite ile Barsak Mikrobiyotası İlişkisi ve Obezitede Prebiyotikler ve Probiyotiklerin Kullanımı. (Relationship Between Obesity and Intestinal Microbiota and Pre-and Probiotics Usage in Obesity). Beslenme ve Diyet Dergisi 2014; 42(2): 148-153.
22. Frankenfield D, Roth-Yousey L, Compher C. Comparison of predictive equations for resting metabolic rate in healthy nonobese and obese adults: a systematic review. J Am Diet Assoc 2005 May;105(5):775-89. doi: 10.1016/j.jada.2005.02.005. PMID: 15883556.
23. Cho S, Dietrich M, Brown CJ, Clark CA, Block G. The effect of breakfast type on total daily energy intake and body mass index: results from the Third National Health and Nutrition Examination Survey (NHANES III). J Am Coll Nutr 2003 Aug;22(4):296-302. doi: 10.1080/07315724.2003.10719307. PMID: 12897044.
24. Pekcan G. Hastanın Beslenme Durumunun Saptanması. Baysal A, Aksoy M, Besler HT, Bozkurt N, Keçecioğlu S, Mercanlıgil SM, Merdol TK, Yıldız E, editors. Diyet El Kitabı. Ankara: Yenilenmiş 6. Baskı, Hatiboğlu Yayınevi; 2011. p. 67-142.
25. Abenavoli L, Scarpellini E, Colica C, et al. Gut Microbiota and Obesity: A Role for Probiotics. Nutrients 2019; 11: 2690. https://doi.org/10.3390/nu11112690.
26. Markowiak P, Śliżewska K. Effects of Probiotics, Prebiotics, and Synbiotics on Human Health. Nutrients 2017; 9(9): 1021. https://doi.org/10.3390/nu9091021.
27. TÜİK, Türkiye Sağlık Araştırması, 2019, Haber Bülteni 2020; Sayı 33661.
28. Sanchez M, Darimont C, Drapeau V, et al. Effect of Lactobacillus rhamnosus CGMCC1.3724 supplementation on weight loss and maintenance in obese men and women. Br J Nutr. 2014 Apr 28;111(8):1507-19. doi: 10.1017/S0007114513003875. Epub 2013 Dec 3. PMID: 24299712.
29. Sharafedtinov KK, Plotnikova OA, Alexeeva RI, et al. Hypocaloric diet supplemented with probiotic cheese improves body mass index and blood pressure indices of obese hypertensive patients--a randomized double-blind placebo-controlled pilot study. Nutr J 2013 Oct 12;12:138. doi: 10.1186/1475-2891-12-138. PMID: 24120179; PMCID: PMC3852723.
30. Rahayu ES, Mariyatun M, Putri Manurung NE, et al. Effect of probiotic Lactobacillus plantarum Dad-13 powder consumption on the gut microbiota and intestinal health of overweight adults. World J Gastroenterol 2021 Jan 7;27(1):107-128. doi: 10.3748/wjg.v27.i1.107. Erratum in: World J Gastroenterol. 2021 Oct 14;27(38):6511-6512. PMID: 33505154; PMCID: PMC7789061.
31. Sohn M, Na GY, Chu J, Joung H, Kim BK, Lim S. Efficacy and Safety of Lactobacillus plantarum K50 on Lipids in Koreans with Obesity: A Randomized, Double-Blind Controlled Clinical Trial. Front Endocrinol (Lausanne) 2022 Jan 19;12:790046. doi: 10.3389/fendo.2021.790046. PMID: 35126309; PMCID: PMC8807682.
32. Majewska K, Kręgielska-Narożna M, Jakubowski H, Szulińska M, Bogdański P. The Multispecies Probiotic Effectively Reduces Homocysteine Concentration in Obese Women: A Randomized Double-Blind Placebo-Controlled Study. J clin med 2020; 9(4): 998. https://doi.org/10.3390/jcm9040998.
33. Rajkumar H, Mahmood N, Kumar M, Varikuti SR, Challa HR, Myakala SP. Effect of probiotic (VSL#3) and omega-3 on lipid profile, insulin sensitivity, inflammatory markers, and gut colonization in overweight adults: a randomized, controlled trial. Mediators of inflammation 2014; 348959. https://doi.org/10.1155/2014/348959.
34. Shavakhi A, Minakari M, Firouzian H, Assali R, Hekmatdoost A, Ferns G. Effect of a Probiotic and Metformin on Liver Aminotransferases in Non-alcoholic Steatohepatitis: A Double Blind Randomized Clinical Trial. Int J Prev Med 2013 May;4(5):531-7. PMID: 23930163; PMCID: PMC3733183.
35. Spaggiari G, Brigante G, De Vincentis S, et al. Probiotics Ingestion Does Not Directly Affect Thyroid Hormonal Parameters in Hypothyroid Patients on Levothyroxine Treatment. Front Endocrinol 2017; 8:316.