Extraction, purification and macrophage stimulatory activity of polysaccharide isolated from leaves of Perilla frutescens BRITTON var. crispa

Main Article Content

Yu Jeong Kim
Jung Soon Hwang
Ki Han Kwon

Keywords

immunostimulatory activity, macrophage, Perilla frutescens BRITTON var. crispa, polysaccharide, purification

Abstract

The crude polysaccharide portion of Perilla frutescens BRITTON var. crispa (PFB-1) was prepared by following steps, hot water extraction (PFB-0), decolorization with methanol reflux and ethanol precipitation. The PFB-1 was separated by anion-exchange column chromatography (fraction: PFB-1-0). Purified PFB-1-0-ii was obtained from gel permeation column chromatography purification steps of PFB-1-0. The PFB-1-0-ii was shown to be one polysaccharide with molecular mass of 10 kDa (kilodalton). PFB-1-0-ii consists of mannose, galactose and glucose as major sugars with 82.8% carbohydrate. Next, this study investigated the effects of PFB-0, PFB-1, PFB-1-0, and PFB-1-0-ii on macrophage stimulatory activity in primary macrophages. PFB-0, PFB-1, PFB-1-0 and PFB-1-0-ii increased macrophage cellular lysosomal enzyme activities. Among the samples tested, PFB-1-0-ii exhibited the highest macrophage lysosomal enzyme activities. The macrophage-stimulating activity was significantly enhanced during the purification step. The results support the utility of purified polysaccharide from Perilla as immunostimulatory activity agent.

Downloads

Download data is not yet available.
Abstract 132 | PDF Downloads 92