Trimmed off adipocytes: a source of newly secreted adiponectin

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Nuraniza Azahari
Muhammad Muzaffar Ali Khan Khattak
Nor Azwani Mohd-Shukri
Suzanah Abdul Rahman
Syed Zahir Idid Syed Osman Idid
Phang Ing Chia
Muhammad Lokman Md Isa
Mohd Aznan Md. Aris
Muhammad Taher

Keywords

Adipose tissue, protein, adiponectin

Abstract

Given the beneficial roles of adiponectin on body metabolism and its profound protective effects against metabolic disease, a better understanding of the adiponectin secretion is very important.  The objective of this study was to isolate, detect and quantify total adiponectin in trimmed off abdominal adipose tissues from halal meat sources namely chicken, beef and lamb. Abdominal adipose tissues were isolated from the aforementioned sources and delipidation of the tissues were performed by chloroform/methanol extractions. Afterwards, the protein concentration was determined by using Protein Assay Bicinchoninate Kit method. This was followed by quantification of the adiponectin using ELISA kit assay. The experiment was conducted in triplicates and the results are presented as means ± SD. The data was statistically analyzed using SPSS (Version 21.0). One-way analysis of variance (ANOVA) was used and the means were considered statistically different at 95% confidence interval. Results indicate that the extraction of 10 gram subcutaneous adipose tissues from chicken, beef and lamb yielded 0.10 gram, 0.15 gram and 0.15 gram respectively of protein amount which was 1 to 1.5 % from total tissue mass. The protein concentration in abdominal adipose tissue from chicken, beef and lamb were 1.25 ± 0.05, 1.75 ± 0.05 & 2.53 ± 0.07 mg/ml, respectively. The isolated adiponectin concentration in chicken, beef and lamb were 158 ng/ml± 0.05, 24 ± 0.05µg/ml and 37 ± 0.08 ng/ml, respectively. Adiponectin concentration in beef abdominal adipose tissue was significantly (p<0.05) higher compared to chicken and lamb. The present study suggests that beef protein have highest amount of adiponectin followed by chicken and lamb. The observed adiponectin proteins in the wasted adipose tissues in meat sources would be promising target for future novel therapeutics in insulin resistance and other metabolic diseases.

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