Osteoporosis prevention in postmenopausal female workers: beneficial effects of silicon dietary supplementation on oxidative status. A pilot study

Main Article Content

Luisella Vigna
Federica De Liso
Laura Tomaino
Giuliana Cighetti
Rita Paroni
Massimo Gestro
Maria Rosaria Ingenito
Filomena Napolitano
Fabrizia Bamonti

Keywords

osteoporosis, osteoporosis prevention, silica supplementation, female workers

Abstract

In the last years, the employment of ageing women is increased, and the well-being of these workers, together with the prevention of chronic disabling diseases, is an issue of great importance. Moreover, as postmenopausal ageing is associated with the loss of bone density and consequent increased fracture risk, promoting bone health in these women could be the best strategy for avoiding osteoporotic fractures. We aimed to evaluate the effects of 3-month supplementation with a commercial antioxidant product containing Silica on oxidative status and bone markers in a sample of Italian female workers. Subjects were menopausal and osteopenic women (N=29, age 59.34±6.37, mean BMI 26.19±4.01 kg/m2). At baseline (T0) and after three-month treatment (T1) bone mineral density (BMD) was evaluated by phalangeal osteosonogrammetry. Haematological, serum biochemical parameters, reactive oxygen species (ROS), total antioxidant capacity (TAC), oxydated low-density lipoproteins (oxLDL) and urinary cross-links pyridinoline (PYD) and deoxypyridinoline (DPD) were assessed. Parametric or non-parametric tests were performed at T0 and T1. To analyse the possible association between two variables a linear correlation test was performed. At T0, slightly high levels of ROS (86% of subjects), oxLDL (59%), Total Cholesterol (T-Chol) (90%) and LDL-Chol (59%) were observed, together with suboptimal or deficient 25-OH vitamin D (98%) concentrations. At T1, oxLDL levels and the ratio oxLDL/LDL-Chol significantly decreased (p<0.01). At T0 significant negative correlations between BMD T-score and cross-links were observed (DPD/Crea: r=-0.57, p=0.001; PYD/ Crea: r=-0.45, p=0.01). At T1, a significant reduction (p=0.03) was observed only for DPD (µg/L) but not for cross-links normalized by creatinine amounts. In conclusion 3-months Silica supplementation improves significantly oxidative status and bone resorption markers in most postmenopausal female workers, representing a complementary treatment for early phases of BMD reduction.

Downloads

Download data is not yet available.
Abstract 65 | PDF Downloads 67

References

(1) Feng X, Mc Donald JM. Disorder of bone remodelling. Annual Review of Patology 2011, 6, 121-145.
(2) Manolagas SC. From estrogen-centric to aging and oxidative stress: a revised perspective of the pathogenesis of osteoporosis. Endocr Rev 2010:31;266-300.
(3) Cauley JA. Public Heath impact of osteoporosis, J. Gerontol. A: Biol. Sci. Med.Sci. 68 (2013) 1243–1251.
(4) Golob AL, Laya MB. Osteoporosis: screening, prevention and management. Med. Clin. North Am. 99 (2015) 587–606.
(5) NIH Consensus Development Panel on Osteoporosis Prevention, Diagnosis, and Therapy. Osteoporosis prevention, diagnosis, and therapy. JAMA 2001; 285:785-795.
(6) Cashman KD. Diet, nutrition, and bone health. J. Nutr 2007; 137: 2507S-2512S.
(7) Jugdaohsingh R, Anderson SH, Tucker KL, et al. Dietary silicon intake and absorption. Am J Clin Nutr. 2002:75: 887-93.
(8) Jugdaohsingh R, Tucker KL, Qiao N, et al. Dietary silicon intake is positively associated with bone mineral density in men and premenopausal woman of the Framingham offspring cohort. J Bone Min Res 2004:19:297-307.
(9) Jugdaohsingh R. Silicon and bone health. J Nutr Health Aging 2007:11:99-110.
(10) Refitt DM, Ogston N, Jugdaohsingh R, et al. Orthosilicic acid stimulates collagen type 1 synthesis and osteoblastic differentiation in human osteoblast-like cells in vitro. Bone 2003: 32: 127-135.
(11) Jurkic LM, Cepanec I, Pavelic SK, Pavelic K. Biological and therapeutic effects of ortho-silicic acid and some ortho-silicic acid-releasing compounds: New perspectives for therapy. Nutrition & Metabolism 2013;10:2-12.
(12) Vigna L, Cassinelli L, Tirelli AS, et al. 25(OH)D Levels in Relation to Gender, Overweight, Insulin Resistance, and Inflammation in a Cross-Sectional Cohort of Northern Italian Workers: Evidence in Support of Preventive Health Care Programs, Journal of the American College of Nutrition, 2017;36(4):253-260.
(13) Soules MR, Sherman S, Parrott E, et al. Executive summary: Stages of Reproductive Aging Workshop (STRAW), Climacteric. 2001;4:4, 267-272, DOI: 10.1080/cmt.4.4.267.272
(14) ATECO (Classification of economic activity) 2007, available at https://www.istat.it/en/ archivio/17959, consulted on July, 2019.
(15) Calvelli L, Accinni R, Gregori D, et al. A quick and effective questionnaire to value dietary intakes and oxidative stress related to food in pneumological patients. Progress in Nutrition 2011;13(1):55-64.
(16) Baroncelli GI. Quantitative ultrasound methods to assess bone mineral status in children: technical characteristics, performance, and clinical application. Pediatric Res. 2008, 63:220-228
(17) Wuster C, Albanese C, De Aloysio D, et al., and The Phalangeal Osteosonogrammetry Study Group. Phalangeal osteosonogrammetry study: age-related changes, diagnostic sensitvity, and discrimination power. J Bone Miner Res 2000, 15, 1603-1614.
(18) Guglielmi G, de Terlizzi F. Quantitative ultrasound in the assessment of osteoporosis. Eur J Radiol 2009, 71, 425-431.
(19) Cighetti G, Bamonti F, Aman CS, et al. Oxidative status in different settings and with different methodological approaches compared by Receiver Operating Characteristic curve analysis. Clin Biochem 2015: 48; 73-78.
(20) De Liso F, Bonara P, Vigna L, et al. Oxidative stress and low-grade inflammatory status as cardiometabolic risk factors in Italian occupational overweight/obese subjects. European Journal of Inflammation. 2013;11:789-796.
(21) Novembrino C, Cighetti G, De Giuseppe R, et al. Effects of encapsulated fruit and vegetable juice powder concentrates on oxidative status in heavy smokers. J Am Coll Nutr 2011: 30; 49-56.
(22) Bamonti F, Pellegatta M, Novembrino C, et al. An encapsulated juice powder concentrate improve markers of pulmonary function and cardiovascular risk factors in heavy smokers. J Am Coll Nutr 2013:32;18-25.
(23) Kraenzlin ME, Kraenzlin CA, Meier C, Giunta C, Steinmann B. Automated HPLC assay for urinary collagen cross-links: effect of age, menopause, and metabolic bone diseases. Clin Chem 2008; 54: 1546-1553.
(24) Monticelli E, Aman CS, Costa ML, et al. Simultaneous free and glycosylated pyridinium crosslink determination in urine: Validation of an HPLC-fluorescence method using a deoxypyridinoline homologue as internal standard. J Chromatogr B 2011; 879: 2764-2771.
(25) Holick MF, Binkley NC, Bischoff-Ferrari HA, et al. and the Endocrine Society: Evaluation, treatment and prevention of vitamin D deficiency: an Endocrine Society clinical practice guideline. J Clin Endocrinol Metab 96:1911–1930, 2011.
(26) Stephan C, Wesseling S, Schink T, Jung K. Comparison of eight computer programs for Receiver-Operatic Characteristic Analysis. Clin Chem 2003: 49: 433-439.
(27) Harris AM, Lanningham-Foster LM, McCrady SK, Levine JA. Nonexercise movement in elderly compared with young people. Am J Physiol Endocrinol Metab 2007 292:E1207–E1212.
(28) Glen KP, Yardley JE, Martineau L, Jay O. Physical Work Capacity in Older Adults: Implications for the Aging Worker. Am J. Industrial Med 2008;51:610–625.
(29) Viguet-Carrin S, Garnero P, Delmas PD. The role of collagen in bone strength. Osteoporosis Int 2006;17:319-336. D. Vashishth. The role of the collagen matrix in skeletal fragility. Current Osteoporosis Reports 2007;5:62-66.
(30) Shoulders MD, Raines RT. Collagen structure and stability. Annu Rev Biochem 2009;78:929- 958.
(31) Roth M, Uebelhart D. Search for new urinary biochemical markers of collagen degradation in man. Clin Chim Acta 2003: 327;81-86.
(32) Leeming DJ, Alexandersen P, Karsdal MA, Qvist P, Schaller S, Tankò LB. An update on biomarkers of bone turnover and their utility in biomedical research and clinical practice. Eur J Clin Pharmacol 2006:62;781-792.
(33) Seibel MJ, Robins SP, Bilezikian JP. Urinary pyridinium cross-links of collagen. Specific markers of bone resorption in metabolic bone disease. Trends Endocrinol Metab 1992;3:263-70.
(34) Garnero P, Piperno M, Gineyts E, Christgau S, Delmas PD, Vignon E. Cross sectional evaluation of biochemical markers of bone, cartilage, and synovial tissue metabolism in patients with knee osteoarthritis: relations with disease activity and joint damage. Ann Rheum Dis 2001;60:619-26.
(35) Baxmann AC, Ahmed MS, Marques NC, et al. Influence of muscle mass and physical activity on serum and urinary creatinine and serum cystatin C. Clin J Am Soc Nephrol 2008; 3: 348-354.
(36) Bernardo I, Bozinovski S, Vlahos R. Targeting oxidant-dependent mechanisms for the treatment of COPD and its comorbidities. Pharmacology &Therapeutics. 2015;155:60-79.
(37) Maziere C, Savitsky V, Galmiche A, Gomila C, Massy Z. Oxidized low density lipoprotein inhibits phosphate signalling and phosphate-induced mineralization in osteoblast. Involvement of oxidative stress. Biochim Biophs Acta 2010;1802:1013-1019.
(38) Lee NK, Choi YG, Baik JY, et al. A crucial role for reactive oxygen species in RANKL-induced osteoclast differentiation. Blood 2005;106: 852-859.
(39) Brodeur MR, Brissette L, Falstrault L, Ouellet P, Moreau R. Influence of oxidized low-density lipoproteins (LDL) on the viability of osteoblastic cells. Free Radic. Biol. Med. 2008;44;506-517.
(40) Fulgenzi A, De Giuseppe R, Bamonti F, Ferrero ME. Improvement of oxidative and metabolic parameters by Cellfood administration in patients affected by neurodegenerative diseases on chelation treatment. BioMed Res Intern, 2014, Article ID 281510, 9 pagesdx.doi.org/10.1155/ 2014/281510).
(41) Mody N, Parhami F, Sarafian TA, Demer LL. Oxidative stress modulates osteoblastic differentiation of vascular and bone cells. Free Radic. Biol. Med. 2001:31;509-519.
(42) Gong HJ, Chen KM, Zhao ZG, Chen CG, Zhou WJ. Effects of silicon on defence of wheat against oxidative stress under drought at different developmental stages. Biol. Plant. 2008; 52: 592-596
(43) Shainkin-Kestenbaum R, Adler AJ, Berlyne GM. Inhibition of superoxide dismutase activity by silicon. J. Trace Elem Electrolytes Health Dis 1990; 4:97-99.
(44) Bellia JP, Birchall JD, Roberts NB. The effect of silicon as silicic acid on superoxide dismutase activity. Clin Chim Acta 1994: 224; 215-217.
(45) Pawlak K, Mysliwiec M, Pawlak D. Oxidized low-density lipoprotein (oxLDL) plasma levels and oxLDL to LDL ratio - are they real oxidative stress markers in dialyzed patients? Life Sci. 2013 Mar 12;92(4-5):253-8. doi: 10.1016/j.lfs.2012.12.002. Epub 2013 Jan 4.
(46) Bolevich S, Kogan AH, Zivkovic V, et al. Protective role of carbon dioxide (CO2) in generation of reactive oxygen species. Mol Cell Biochem 2016;411:317-330.
(47) Omodei U, Benussi C, Ramazzotto F, Feller M, Gambacciani M. Risk identification of osteoporosis in post-menopausal women by a simple algorithm based on ultrasound densitometry and body mass index. J Clin Densitom. 2008;11: 412-416.
(48) Albanese CV, Cepollaro C, De Terlizzi F, Brandi ML, Passariello R. Performance of five phalangeal QUS parameters in the evaluation of gonadal-status, age and vertebral fracture risk compared with DXA. Ultrasound in Med & Biol, 2009;35:537-544.