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intestinal microbiota, diversity, exercise, nnutrition
Aim: The aim of this study is to determine whether exercise and nutrition style has an effect on the intestinal microbiota diversity. Materials and Method: 15 healthy male volunteers between the ages of 18 and 24 participated in the study and they were grouped in three as professional athletes (n=5), amateur athletes (n=5) and sedentary individuals (n=5). “Stool samples” were taken from the participants once to find out their intestinal microbiota diversity, metagenomics analysis was conducted with New Generation Sequencing Method by using Illumina MiSeq analyzer and “Nutrient Consumption” questionnaire was given to find out their nutritional habits. Minitab 17 and SPSS 20.0 programs were used for the statistical analysis of the results and significance level was taken as p<0.05. Results:In our study which analyzed the species diversity, according to Shannon index, it was found that the group with the highest species diversity was the professional athletes group, while the group with the lowest species diversity was the sedentary individuals group. According to the Phylo diversity index, the group with the most phylo diversity was the professional athletes group, while the group with the least species diversity was the sedentary individuals group and a significant difference was found between amateur-professional, amateur-sedentary and professional-sedentary groups in terms of phylogenetic diversity (p<0.05). In addition, it was found that professional athletes had a diet consisting of protein predominantly, amateur athletes had a diet consisting of carbohydrates predominantly and sedentary individuals had a diet consisting of vegetable-fruit predominantly.Conclusion: it is thought that exercise influences intestinal microbiota diversity positively and different diets also have an influence in different microbial diversity between groups.
Key Words: Intestinal microbiota, Diversity, Exercise, Nutrition
2. Mika A, Fleshner M. Early-life exercise may promote lasting brain and metabolic health through gut bacterial metabolites. Immunology and Cell Biology, 2016; 94(2): 151.
3. Perlmutter D. Loberg K. Brain and intestine. İstanbul: Pegasus Publications 2017.
4. Mayer E. Brain-Intestine Connection. İstanbul: Paloma Publisher 2017.
5. Clarke SF, Murphy EF, O’Sullivan O et al. Exercise and associated dietary extremes impact on gut microbial diversity. Gut, 2014; 63:1913–1920.
6. Bilici MF, Güler MŞ. The Investigation of the Acute Effect of Sparring Training on Some biochemical parameters in Elite Boxers. International Journal of Applied Exercise Physiology, 2019; 8(3.1):357-360.
7. Monda V, Villaono I, Messina A et al. Exercise Modifies the Gut Microbiota with Positive Health Effects. Oxidative Medicine and Cellular Longevity, 2017;1-8.
8. Mach N, Fuster-Botella D. Endurance exercise and gut microbiota: A review. Journal of Sport and Health Science, 2017; 6(2):179-197.
9. Yatsunenko T, Rey FE, Manary MJ et al. Human gut microbiome viewed across age and geography. Nature, 2012; 486(7402): 222–227.
10. Singh A, Zapata RC, Pezeshki A, Reidelberger RD, Chelikani PK. Inulin ﬁber dose-dependently modulates energy balance, glucose tolerance, gut microbiota, hormones and diet preference in high-fat-fed male rats. J. Nutr. Biochem, 2018; 59:142–152.
11. Edgar Robert C et al. "UCHIME improves sensitivity and speed of chimera detection." Bioinformatics, 2011; 2194-2200.
12. DP Faith. Conservation evaluation and phylogenetic diversity. Biological Conservation, 1992; 61: 1-10.
13. Bressa C, Andrino AB, Santiago JP et al. Differences in gut microbiota profile between women with active lifestyle and sedentary women. PLOS ONE, 2017; 12(2):1-20.
14. Choie JJ, Eum SY, Rampersaud E, Daunert S, Abreu MT, Toborek M. Exercise attenuates PCB-induced changes in the mouse gut microbiome. Environ Health Perspect, 2013;121:725–730.
15. Codella R, Luzi L, Terruzzi I. Exercise has the guts: How physical activity may positively modulate gut microbiota in chronic and immune-based diseases. Digestive and Liver Disease, 2017;1-48.
16. Mika A, Fleshner M. Early-life exercise may promote lasting brain and metabolic health through gut bacterial metabolites. Immunology and cell biology, 2016;1-9.
17. Šket R, Treichel N, Kublik S et al . Hypoxia and inactivity related physiological changes precede or take place in absence of significant rearrangements in bacterial community structure: The PlanHab randomized trial pilot study. PLOS ONE, 2017; 12(12): 0188556.
18. Allen JM, Berg Miller, ME, Pence BD, Whitlock K, NehraV, Gaskins HR, White BA, Fryer JD, Woods JA.Voluntary and forced exercise differentially alters the gut microbiome in C57BL/6J mice. Journal of Applied Physiology, 2015; 118(8):1059-1066.
19. Hamasaki H. Exercise and gut microbiota: clinical implications for the feasibility of Tai Chi. Journal of Integrative Medicine, 2017; 15(4): 270-282.
20. Houghton D, Stewart DJ, Day DP, Trenell M. Gut Microbiota and Lifestyle Interventionsin NAFLD. International Journal of Molecular Sciences, 2016;17(447):2-29.
21. Barton W, Penney NC, Cronin O, Garcia-Perez I, Molloy MG, Holmes E, Shanahan F, Cotter PD O’Sullivan O. The microbiome of professional athletes differs from that of more sedentary subjects in composition and particularly at the functional metabolic level. Gut, 2018; 67(4):625-633.
22. Kang SS, Jeraldo PR, Kurti A et al. Diet and exercise orthogonally alter the gut microbiome and reveal independent associations with anxiety and cognition. Molecular Neuro degeneration, 2014; 9(1): 36.
23. Estaki M, Pither J, Baumeister P, Little JP, Gill SK, Ghosh S, Vand ZA, Marsden KT, Gibson, DL Cardiorespiratory fitness as a predictor of intestinal microbial diversity and distinct metagenomic functions. Microbiome, 2016;4(42): 2-13.
24.Genç A, Tutkun E, Acar H, Zorba E. Investigation of Relation Between Clostridium Colonization and Nutrient Consumption in Intestinal Flora in Athletes and Sedentary Men. Progr Nutr [Internet]. 2019Apr.4 [cited 2019Sep.5] ;22(2).
25. Sonnenburg ED, Smits SA, Tikhonov M, Higginbottom SK, Wingreen NS, Sonnenburg JL. Diet-induced extinctions in the gut microbiota compound over generations. Nature, 2016, 529(7585): 212.
26. Fillippis FD, Pellegrini N, Vannini L et al. High-level adherence to a Mediterranean diet beneficially impacts the gut microbiota and associated metabolome. Gut, 2015; 65(11): 1812-1821.
27. Shin JH, Sim M, Lee JY, Shin DM. Lifestyle and geographic insights into the distinct gut microbiota in elderly women from two different geographic locations. Journal of Physiological Anthropology, 2016; 35(31): 2-9.
28. Campbell S, Wisniewski PJ. Exercises is a novel promoter of intestinal health and microbial diversity. Exercise and Sports Sciences, 2017;45(1): 41-47.
29. Wu J, Xu S, Xiang C, Cao Q, Li Q, Huang J, Shi L, Zhang J, Zhan Z .Tongue coating microbiota community and risk effect on gastric cancer. Journal of Cancer, 2018; 9(21): 4039-4048.
30. Cani PD, Possemiers S, Van de Wiele T et al. Changes in gut microbiota control inﬂammation in obese mice through a mechanism involving GLP-2-driven improvement of gut permeability. Gut, 2009;58: 1091–1103.
31. Kopf JC, Suhr MJ, Clarke J, Eyun SI, Riethoven JM, Ramer-Tait AE, Rose DJ. Role of whole grains versus fruits and vegetables in reducing subclinical inﬂammation and promoting gastrointestinal health in individuals affected by overweight and obesity: A randomized controlled trial. Nutr. J., 2018; 17:72.