Determination of the Promoting Role of Oleaster Fruit in Protein Synthesis by SDS-PAGE and Some Biochemical Analysis

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Ozlem Gok
Seda Beyaz
Serpil Baspinar
Abdullah Aslan


Copper chloride, Oleaster fruit, Protein, Saccharomyces cerevisiae, SDS-PAGE.


Study Objective: Oleaster fruit (Elaeagnus angustifolia) have been used in traditional medicine to treat various diseases. In this study, the effect of oleaster fruit extract on oxidative damage in culture of Saccharomyces cerevisiae (S.cerevisiae) caused by copper chloride (CuCl2) was investigated.

Methods: In this study, 4 groups were formed. Groups; (i) Control group; (ii) Oleaster Fruit Group (8%); (iii) Copper chloride (CuCl2) Group (30 mM); (iv) Oleaster Fruit (8%) + Copper chloride (CuCl2) (30 mM) Group. S. cerevisiae cultures were grown at 30 °C for 1 hour, 3 hours, 5 hours and 24 hours. Cell growth, lipid peroxidation, MDA (malondialdehyde) analyzes, glutathione (GSH) levels and catalase activities were determined by spectrophotometer. Total protein changes were detected by SDS-PAGE electrophoresis and calculated by the Lowry method.

Results: According to the results obtained; oleaster fruit extract added to S.cerevisiae cultures showed cell growth (1, 3, 5 and 24 hours), total protein synthesis (1 hour, 3 hours, 5 hours and 24 hours), GSH levels (24 hours) and catalase activities (24 hours) and decreased the MDA level (24 hours).

Conclusions: These results show that the oleaster fruit extract reduces oxidative damage, enhances cell growth and has a protective effect to promote protein synthesis in S.cerevisiae culture.

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1. Farzaei MH, Bahramsoltani R, Abbasabadi Z, Rahimi RA Comprehensive Review on Phytochemical And Pharmacological Aspects of Elaeagnus angustifolia L. J Pharm Pharmacol 2015; 67(11): 1467-1480.
2. Sahan Y, Aydin E, Dundar AI, Altiner DD, Celik G, Gocmen D. Effects of Oleaster Flour Supplementation in Total Phenolic Contents, Antioxidant Capacities And Their Bioaccessibilities of Cookies. Food Sci Biotechnol 2019; 28(5): 1401-1408.
3. Amereh Z, Hatami N, Shirazi FH, Gholami S, Hosseini SH, Noubarani M, et al. Cancer Chemoprevention by Oleaster (Elaeagnus angustifoli L.) Fruit Extract in a Model of Hepatocellular Carcinoma Induced by Diethylnitrosamine in Rats. EXCLI J 2017; 16: 1046.
4. Hamidpour R, Hamidpour S, Hamidpour M, Shahlari M, Sohraby M, Shahlari N, Hamidpour R. Russian Olive (Elaeagnus angustifolia L.): From a Variety of Traditional Medicinal Applications to Its Novel Roles As Active Antioxidant, Anti-Inflammatory, Anti-Mutagenic And Analgesic Agent. J Tradit Complement Med 2017; 7(1): 24-29.
5. Gao P, Xu W, Yan T, Zhang C, Lv X, He Y. Application of Near-Infrared Hyperspectral Imaging with Machine Learning Methods to Identify Geographical Origins of Dry Narrow-Leaved Oleaster (Elaeagnus angustifolia) Fruits. Foods 2019; 8(12): 620.
6. Karademir BÇ. Molecular Characterization of Russian Olive (Elaeagnus Angustifolia L.) Genotypes Grown in Kayseri Province. Ordu University Ordu Turkey 2015.
7. Herrero E, Ros J, Bellí G, Cabiscol E. Redox Control And Oxidative Stress in Yeast Cells. Biochimic Biophys Acta (BBA)-Gen Subject 2008; 1780(11): 1217-1235.
8. Odriozola-Serrano I, Puigpinós J, Oliu GO, Herrero E, Martín-Belloso O. Antioxidant Activity of Thermal or Non-Thermally Treated Strawberry And Mango Juices by Saccharomyces cerevisiae Growth Based Assays. LWT 2016; 74: 55-61.
9. Aslan A, Can MI. Protein Expression Product Alterations in Saccharomyces cerevisiae. Progress in Nutrition 2017; 19 (1): 81-85.
10. Aslan A, Gok O, Beyaz S. Protective effect of grape seed extract against hydrogen peroxide damage created in Saccharomyces cerevisiae. Igdır Univ J Inst Sci Tech. 2019a; 9(4): 2216-2224.
11. Aslan A, Gok O, Erman O. The protective effect of kiwi fruit extract against to chromium effect on protein expression in Saccharomyces cerevisiae. Prog Nutr. 2017; 19 (4): 472-476.
12. Aslan A, Beyaz S, Gok O. The protective effect of tomato extract against to chromium-induced damage in Saccharomyces cerevisiae. Univ J Sci Tech. 2019b; 12(2): 1048-1055.
13. Laemmli U.K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, 227: 680-685.
14. Aslan A, Gok O, Erman O, Kuloglu T. Ellagic acid impedes carbontetrachloride-induced liver damage in rats through suppression of NF-kB, Bcl-2 and regulating Nrf-2 and caspase pathway. Biomed Pharmacother. 2018; 105: 662-669.
15. Merkit C. Investigation of the effect of β-carotene on nitric oxide and malondialdehyde levels in rabbits given diethylnitrosamine. Kafkas Univ Turkey Kars 2018.
16. Erol C. Examination of the activities of serum malondialdehyde acid, superoxide dismutase, catalase, reduced glutation and glutation peroxidase in acute romatic fevered patients. Van Yüzüncü Yıl Univ Van Turkey 2020.
17. Aslan A, Gok O, Beyaz S, Ağca CA, Erman O, Zerek A. Ellagic acid prevents kidney injury and oxidative damage via regulation of Nrf-2/NF-κB signaling in carbon tetrachloride induced rats. Mol Rep. 2020a; 47(10): 7959-7970.
18. Al-Attar AM, Alrobai AA, Almalki DA. Effect of Olea oleaster and Juniperus Procera Leaves Extracts on Thioacetamide Induced Hepatic Cirrhosis in Male Albino Mice. Saudi J Biol Sci 2016; 23(3): 363-371.
19. Beyaz S, Dalkılıç LK, Gök Ö, Aslan A. Effect of Black Mulberry (Morus nigra L.) and Cranberry (Cornus mas L.) on some molecular biological and biochemical parameters against oxidative damage caused by hydrogen peroxide in Saccharomyces cerevisiae. BEU Journal of Science. 2020; 9(3): 1134-1144.
20. Aslan A, Boydak D, Can MI, Kuloğlu T. Nigella sativa Improves the Carbon Tetrachloride-Induced Lung Damage in Rats Through Repression of Erk/Akt Pathway. Bangladesh J. Pharmacol 2015; 10: 654-659.
21. Amini N, Sarkaki A, Dianat M, Mard SA, Ahangarpour A, Badavi M. Protective Effects of Naringin And Trimetazidine on Remote Effect of Acute Renal Injury on Oxidative Stress And Myocardial Injury Through Nrf-2 Regulation. Pharmacol Rep 2019; 71(6): 1059-1066.
22. Aslan A, Beyaz S, Gok O, Can MI, Erman F, Erman O. The impact of ellagic acid on some apoptotic gene expressions: a new perspective for the regulation of pancreatic Nrf-2/NF-κB and Akt/VEGF signaling in CCl4-induced pancreas damage in rats. Immunopharmacol Immunotoxicol 2020b; 1-8.
23. Aslan A, Beyaz S, Gok O, Erman O. The effect of ellagic acid on caspase-3/bcl-2/Nrf-2/NF-kB/TNF-α /COX-2 gene expression product apoptosis pathway: a new approach for muscle damage therapy. Mol Biol Rep. 2020c; 47:2573–2582.
24. Motevalian M, Shiri M, Shiri S, Shiri Z, Shiri H. Anti-inflammatory activity of Elaeagnus angustifolia fruit extract on rat paw edema. J Basic Clin Physiol Pharmacol 2017; 28(4): 377-381.
25. Dhingra D, Jangra A. Antiepileptic activity of ellagic acid, a naturally occurring polyphenolic compound, in mice. J Funct Foods 2014; 10: 364–369.
26. Guan Y, Cui ZJ, Sun B, Han LP, Li CJ, Chen LM. Celastrol attenuates oxidative stress in the skeletal muscle of diabetic rats by regulating the AMPK-PGC1α-SIRT3 signaling pathway. Int J Mol Med 2016; 37 (5). 1229-1238.
27. Luo KW, Chen W, Lung WY, Wei XY, Cheng BH, Cai ZM, et al. EGCG inhibited bladder cancer SW780 cell proliferation and migration both in vitro and in vivo via down-regulation of NF-κB and MMP-9. J Nutr Biochem 2017; 41: 56–64.
28. Aslan A. The Effects of Different Essential Fj and Their Combination on Saccharomyces cerevisiae Cell Growth. Progr Nutr 2015; 17(1): 36-40.
29. Aslan A, Baspinar S, Yilmaz O. Is Pomegranate Juice Has A Vital Role for Protective Effect on Saccharomyces cerevisiae Growth?. Progr Nutr 2014; 16 (3): 212-217.
30. Gök A. The Effects Indole-3-Acetic Acid, Gibberellic Acid And 6-Furfurilaminopurin Mixtures on Cambial Activity in Oleaster Cuttġngs (Elaeagnus Angustifolia L.) Exposed to Lead Stress. Yüzüncü Year University Institute Of Science Van Turkey 2010.
31. Jilani H, Cilla A, Barberá R, Hamdi M. Improved Bioaccessibility and Antioxidant Capacity of Olive Leaf (Olea europaea L.) Polyphenols Through Biosorption on Saccharomyces cerevisiae. Ind Crops Prod 2016; 84: 131-138.
32. Dinda B, Kyriakopoulos AM, Dinda S, Zoumpourlis V, Thomaidis NS, Velegraki A, Markopoulos C, Dinda M. Cornus mas L.(cornelian cherry), an Important European and Asian Traditional Food and Medicine: Ethnomedicine, Phytochemistry and Pharmacology for its Commercial Utilization in Drug Industry. J Ethnopharmacol 2016; 193:670-690.
33. Hosseinzadeh H, Ramezani M, Namjo N. Muscle relaxant activity of Elaeagnus angustifolia L. fruit seeds in mice. J Ethnopharmacol 2003; 84(2-3): 275-278.
34. Aslan A, Gok O, Beyaz S, Arslan E, Erman O, Ağca CA. The preventive effect of ellagic acid on brain damage in rats via regulating of Nrf‐2, NF‐kB and apoptotic pathway. J Food Biochem. 2020e; 44(6): e13217.
35. Saleh AI, Mohamed I, Mohamed AA, Abdelkader M, Yalcin HC, Aboulkassim T. Elaeagnus angustifolia plant extract inhibits angiogenesis and downgrades cell invasion of human oral cancer cells via erk1/Erk2 inactivation. Nutr Cancer 2018; 70(2): 297-305.
36. Aslan A, Can MI. The inhbition of chromium effect in Saccharomyces cerevisiae thrive from grapefruit. Prog Nutr 2015a; 17(4): 339-342.
37. Aslan A, Can MI. The effect of orange juice against to H2O2 stress in Saccharomyces cerevisiae. Progr Nutr 2015b; 17(3): 250-254.

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