Effects of (+)- catechin + quercetin usage before exhaustion exercise on free radical and antioxidant enzyme levels (+)- catechin + quercetin usage before Exhaustion Exercise

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Erdil Durukan


Antioxidants, Catechin, Exhaustion Exercise, Free Radicals, Quercetin


Study Objectives: The aim of this study was to determine the effects of the use of (+) -Catechin + Quercetin for 10 days before an exhaustion exercise on free radical and antioxidant enzyme levels. Methods: The study was performed on 12 male Wistar rats (260-320 gr.) from the same family and animals divided into two groups as a control and experimental group. After the first exhaustion exercise, rats in the experimental group used (+)- Catechin + Quercetin in addition to the standard laboratory diet and they performed the second exhaustion exercise. In the experimental group, 20 mg / kg (+) - Catechin + Quercetin substances were dissolved in dimethyl sulfoxide and given 1 ml / kg while the control group received 1 ml / kg 0.05% dimethyl sulfoxide by gavage in addition to standard laboratory diet daily for 10 days. SOD, CAT, GPx, GST, and MDA levels were measured by spectrophotometer. The IBM SPSS Statistics 24.0 was preferred for the statistical analysis. The repeated measures two-factor variance analysis was used to determine the difference between control and experimental groups. Results: It was determined that antioxidant enzyme levels (SOD, CAT, GPx, and GST levels) in rats using (+) - Catechin + Quercetin for 10 days before an exhaustion exercise were higher than those of the control group. Despite that, it was determined that MDA levels in rats using (+) - Catechin + Quercetin for 10 days before an exhaustion exercise were lower than those of the control group. Conclusion: It can be said that the use of (+)- Catechin + Quercetin can reduce the amount of MDA which is the end product of lipid peroxidation in exercise and may create a protective effect against free radicals and increase the levels of antioxidant enzymes and strengthen the antioxidant defense systems of the cells and have a positive effect on exercise performance.

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1- Yagmur, R., Isik, O., Kilic, Y., & Akyuz, M. (2019). The hydration status and thyroid hormones levels among elite wrestlers. Progress in Nutrition, 21(4).
2- Ji, L. L. (1999). Antioxidants and oxidative stress in exercise. Proceedings of the Society for experimental Biology and Medicine, 222(3), 283-292.
3- Ji, L. L., Leeuwenburgh, C., Leichtweis, S., Gore, M., Fiebig, R., Hollander, J., & Bejma, J. (1998). Oxidative stress and aging: role of exercise and its influences on antioxidant systems. Annals of the New York Academy of Sciences, 854(1), 102-117.
4- König, D., Wagner, K. H., Elmadfa, I., & Berg, A. (2001). Exercise and oxidative stress: significance of antioxidants with reference to inflammatory, muscular, and systemic stress. Exercise immunology review, 7, 108-133.
5- Packer, L. (1997). Oxidants, antioxidant nutrients and the athlete. Journal of sports sciences, 15(3), 353-363.
6- Urso, M. L., & Clarkson, P. M. (2003). Oxidative stress, exercise, and antioxidant supplementation. Toxicology, 189(1-2), 41-54.
7- Selçuk, M. (2003). Sedanterler ile kuzey disiplini yapan antrene bireylerde programlı aerobik ve anaerobik egzersizlerin bazı antioksidan profiller üzerine etkilerinin araştırılması. Sağlık Bilimleri Enstitüsü, Fizyoloji Anabilim Dalı, Doktora Tezi, Yüzüncü Yıl Üniversitesi, Van, Türkiye.
8- Halliwell, B., & Aruoma, O. I. (1991). DNA damage by oxygen-derived species Its mechanism and measurement in mammalian systems. FEBS letters, 281(1-2), 9-19.
9- Formica, J. V., & Regelson, W. (1995). Review of the biology of quercetin and related bioflavonoids. Food and chemical toxicology, 33(12), 1061-1080.
10- Rice-Evans, C. A., Miller, N. J., & Paganga, G. (1996). Structure-antioxidant activity relationships of flavonoids and phenolic acids. Free radical biology and medicine, 20(7), 933-956.
11- Hayek, T., Fuhrman, B., Vaya, J., Rosenblat, M., Belinky, P., Coleman, R., ... & Aviram, M. (1997). Reduced progression of atherosclerosis in apolipoprotein E–deficient mice following consumption of red wine, or its polyphenols quercetin or catechin, is associated with reduced susceptibility of LDL to oxidation and aggregation. Arteriosclerosis, thrombosis, and vascular biology, 17(11), 2744-2752.
12- Çimen, M. B. Y. (1999). Flavonoidler ve antioksidan özellikleri. Turkiye Klinikleri Journal of Medical Sciences, 19(5), 296-304.
13- Husain, S. R., Cillard, J., & Cillard, P. (1987). Hydroxyl radical scavenging activity of flavonoids. Phytochemistry, 26(9), 2489-2491.
14- Kumari, M. R., Yoneda, T., & Hiramatsu, M. (1997). Effect of “βCATECHIN” on the life span of senescence accelerated mice (SAM‐P8 strain). IUBMB Life, 41(5), 1005-1011.
15- Rice-Evans, C. (1995, November). Plant polyphenols: free radical scavengers or chain-breaking antioxidants?. In Biochemical Society Symposia (Vol. 61, pp. 103-116). Portland Press Limited.
16- De Whalley, C. V., Rankin, S. M., Hoult, J. R. S., Jessup, W., & Leake, D. S. (1990). Flavonoids inhibit the oxidative modification of low density lipoproteins by macrophages. Biochemical pharmacology, 39(11), 1743-1750.
17- Morel, I., Lescoat, G., Cillard, P., & Cillard, J. (1994). [43] Role of flavonoids and iron chelation in antioxidant action. In Methods in enzymology (Vol. 234, pp. 437-443). Academic Press.
18- Yu, F. R., Liu, Y., Cui, Y. Z., Chan, E. Q., Xie, M. R., McGuire, P. P., & Yu, F. H. (2010). Effects of a flavonoid extract from Cynomorium songaricum on the swimming endurance of rats. The American journal of Chinese medicine, 38(01), 65-73.
19- Kocabaş, N. (2008). Homosisteinin indüklediği oksidatif stres üzerinde quercetinin koruyucu etkisi. Master's thesis, Afyon Kocatepe Üniversitesi, Sağlık Bilimleri Enstitüsü, Afyon, Türkiye.
20- Duarte, J., Pérez‐Palencia, R., Vargas, F., Angeles Ocete, M., Pérez‐Vizcaino, F., Zarzuelo, A., & Tamargo, J. (2001). Antihypertensive effects of the flavonoid quercetin in spontaneously hypertensive rats. British journal of pharmacology, 133(1), 117-124.
21- Adewole, S. O., Caxton-Martins, E. A., & Ojewole, J. A. (2007). Protective effect of quercetin on the morphology of pancreatic β-cells of streptozotocin-treated diabetic rats. African Journal of Traditional, Complementary and Alternative Medicines, 4(1), 64-74.
22- Agarwal, R., Katiyar, S. K., Khan, S. G., & Mukhtar, H. (1993). Protection against ultraviolet B radiation‐induced effects in the skin of SKH‐1 hairless mice by a polyphenolic fraction isolated from green tea. Photochemistry and photobiology, 58(5), 695-700.
23- Akyüz, Ö. (2016). Effects of Different-Term Acute Swimming Exercises on Oxidative Stress Parameters in Rat Cerebellum Tissue. US-China Education Review B, 6(10), 600-608.
24- Demirci, N. (2017). Protective Effect of Prolonged Quercetin Supplement on Oxidative Stress and Antioxidant Enzyme Activities in Boxers. Studies on Ethno-Medicine, 11(2), 168-173.
25- Sadowska-Krępa, E., Domaszewski, P., Pokora, I., Żebrowska, A., Gdańska, A., & Podgórski, T. (2019). Effects of medium-term green tea extract supplementation combined with CrossFit workout on blood antioxidant status and serum brain-derived neurotrophic factor in young men: a pilot study. Journal of the International Society of Sports Nutrition, 16(1), 1-9.
26- Park, H. J., DiNatale, D. A., Chung, M. Y., Park, Y. K., Lee, J. Y., Koo, S. I., ... & Bruno, R. S. (2011). Green tea extract attenuates hepatic steatosis by decreasing adipose lipogenesis and enhancing hepatic antioxidant defenses in ob/ob mice. The Journal of nutritional biochemistry, 22(4), 393-400.
27- Phachonpai, W., Wattanathorn, J., Muchimapura, S., Tong-Un, T., & Preechagoon, D. (2010). Neuroprotective effect of quercetin encapsulated liposomes: a novel therapeutic strategy against Alzheimer's disease. American Journal of Applied Sciences, 7(4), 480-485.
28- Vassalle, C., Pingitore, A., De, R. G., Vigna, L., & Bamonti, F. (2015). Antioxidants in Sport Nutrition. In Biomarkers Part II: Biomarkers to estimate bioefficacy of dietary/supplemental antioxidants in sport. CRC Press/Taylor & Francis.
29- Göktepe, M., & Günay, M. (2014). Quercetin uygulamasının egzersiz, serbest radikal ve antioksidan enzim düzeyleri üzerine etkisi. International Journal of Sport Culture and Science, 2(Special Issue 1), 775-788.
30- Durukan, E. (2019). Egzersizdeki Serbest Radikal ve Antioksidan Enzim Düzeylerine Catechin Uygulamasının Etkisi, Gazi Kitabevi, Ankara.
31- Dresser, G. K., Bailey, D. G., Leake, B. F., Schwarz, U. I., Dawson, P. A., Freeman, D. J., & Kim, R. B. (2002). Fruit juices inhibit organic anion transporting polypeptide–mediated drug uptake to decrease the oral availability of fexofenadine. Clinical pharmacology & therapeutics, 71(1), 11-20.
32- Malien-Aubert, C., Dangles, O., & Amiot, M. J. (2001). Color stability of commercial anthocyanin-based extracts in relation to the phenolic composition. Protective effects by intra-and intermolecular copigmentation. Journal of agricultural and food chemistry, 49(1), 170-176.
33- Ohtani, M., Kawabata, S., Kariya, S., Uchino, K., Itou, K., Kotaki, H., ... & Nishida, N. (2002). Effect of grapefruit pulp on the pharmacokinetics of the dihydropyridine calcium antagonists nifedipine and nisoldipine. Yakugaku zasshi: Journal of the Pharmaceutical Society of Japan, 122(5), 323-329.
34- Silberberg, M., Morand, C., Manach, C., Scalbert, A., & Remesy, C. (2005). Co-administration of quercetin and catechin in rats alters their absorption but not their metabolism. Life sciences, 77(25), 3156-3167.
35- Violi, F., Pignatelli, P., & Pulcinelli, F. M. (2002). Synergism among Flavonoids in Inhibiting Platelet Aggregation and H. Circulation, 105, e53.
36- Drabkin, D. L. (1946). Spectrophotometric studies XIV. The crystallographic and optical properties of the hemoglobin of man in comparison with those of other species. Journal of biological chemistry, 164(2), 703-723.
37- Marklund, S., & Marklund, G. (1974). Involvement of the superoxide anion radical in the autoxidation of pyrogallol and a convenient assay for superoxide dismutase. European journal of biochemistry, 47(3), 469-474.
38- Aebi, H. (1984). Catalase in vitro. In Methods in enzymology, 105, 121-126
39- Paglia, D. E., & Valentine, W. N. (1967). Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase. The Journal of laboratory and clinical medicine, 70(1), 158-169.
40- Habig, W. H., Pabst, M. J., & Jakoby, W. B. (1974). Glutathione S-transferases the first enzymatic step in mercapturic acid formation. Journal of biological Chemistry, 249(22), 7130-7139.
41- Ohkawa, H., Ohishi, N., & Yagi, K. (1979). Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Analytical biochemistry, 95(2), 351-358.
42- Isik, O., & Dogan, I. (2018). Effects of bilateral or unilateral lower body resistance exercises on markers of skeletal muscle damage. biomedical journal, 41(6), 364-368.
43- Koçyiğit, A., Arslan, S. O., Erel, Ö., Aktepe, N., Avcı, Ş., & Gür, S. (2000). Effects of dietary supplementation of catechin on antioxidantenzyme activities and lipid peroxidation levels in rats. Turkiye Klinikleri Journal of Medical Sciences, 20(1), 1.
44- Anjaneyulu, M., & Chopra, K. (2003). Quercetin, a bioflavonoid, attenuates thermal hyperalgesia in a mouse model of diabetic neuropathic pain. Progress in neuro-psychopharmacology and biological psychiatry, 27(6), 1001-1005.
45- Abdallah, F. B., Fetoui, H., Fakhfakh, F., & Keskes, L. (2012). Caffeic acid and quercetin protect erythrocytes against the oxidative stress and the genotoxic effects of lambda-cyhalothrin in vitro. Human & experimental toxicology, 31(1), 92-100.
46- Molina, M. F., Sanchez-Reus, I., Iglesias, I., & Benedi, J. (2003). Quercetin, a flavonoid antioxidant, prevents and protects against ethanol-induced oxidative stress in mouse liver. Biological and Pharmaceutical Bulletin, 26(10), 1398-1402.
47- Nabavi, S. F., Eslami, S., Moghaddam, A. H., Jafari, N., Nabavi, S. M., & Ebrahimzadeh, M. A. (2011). Protective effects of quercetin against sodium fluoride-induced oxidative stress in rat erythrocytes. Toxicological & Environmental Chemistry, 93(8), 1666-1675.
48- Devi, P. S., & Shyamala, D. C. (1999). Protective effect of quercetin in cisplatin-induced cell injury in the rat kidney. Indian journal of pharmacology, 31(6), 422-426.
49- Kahraman, A., Erkasap, N., Köken, T., Serteser, M., Aktepe, F., & Erkasap, S. (2003). The antioxidative and antihistaminic properties of quercetin in ethanol-induced gastric lesions. Toxicology, 183(1-3), 133-142.
50- Middleton, E., Kandaswami, C., & Theoharides, T. C. (2000). The effects of plant flavonoids on mammalian cells: implications for inflammation, heart disease, and cancer. Pharmacological reviews, 52(4), 673-751.
51- Nijveldt, R. J., Van Nood, E. L. S., Van Hoorn, D. E., Boelens, P. G., Van Norren, K., & Van Leeuwen, P. A. (2001). Flavonoids: a review of probable mechanisms of action and potential applications. The American journal of clinical nutrition, 74(4), 418-425.
52- Uygur, R., Yagmurca, M., Alkoc, O. A., Genc, A., Songur, A., Ucok, K., & Ozen, O. A. (2014). Effects of quercetin and fish n‐3 fatty acids on testicular injury induced by ethanol in rats. Andrologia, 46(4), 356-369.
53- Behling, E. B., Sendão, M. C., Francescato, H. D., Antunes, L. M., Costa, R. S., & Bianchi, M. D. L. P. (2006). Comparative study of multiple dosage of quercetin against cisplatin-induced nephrotoxicity and oxidative stress in rat kidneys. Pharmacological reports, 58, 526-532.
54- Çiftçi, R. (2013). Karaciğer fibrozisli ratlarda kuersetinin homosistein düzeyi ve koroner damar hasarı üzerine etkisi, Fırat Üniversitesi Sağlık Bilimleri Enstitüsü Fizyoloji Anabilim Dalı, Yükseklisans Tezi, Elazığ, Türkiye.
55- Hollman, P. C., de Vries, J. H., van Leeuwen, S. D., Mengelers, M. J., & Katan, M. B. (1995). Absorption of dietary quercetin glycosides and quercetin in healthy ileostomy volunteers. The American journal of clinical nutrition, 62(6), 1276-1282.
56- Sriraksa, N., Wattanathorn, J., Muchimapura, S., Tiamkao, S., Brown, K., & Chaisiwamongkol, K. (2012). Cognitive-enhancing effect of quercetin in a rat model of Parkinson's disease induced by 6-hydroxydopamine. Evidence-Based Complementary and Alternative Medicine, 1-9.
57- Chander, V., Singh, D., & Chopra, K. (2003). Catechin, a natural antioxidant protects against rhabdomyolysis-induced myoglobinuric acute renal failure. Pharmacological research, 48(5), 503-509.
58- Gul, M., Kutay, F. Z., Temocin, S., & Hanninen, O. (2000). Cellular and clinical implication of glutathione. Indian Journal of Experimental Biology, 38(7), 625-634.
59- Abarikwu, S. O., Pant, A. B., & Farombi, E. O. (2012). Dietary antioxidant, quercetin, protects sertoli‐germ cell coculture from atrazine‐induced oxidative damage. Journal of biochemical and molecular toxicology, 26(11), 477-485.
60- Aguirre, L., Arias, N., Teresa Macarulla, M., Gracia, A., & P Portillo, M. (2011). Beneficial effects of quercetin on obesity and diabetes. The Open Nutraceuticals Journal, 4(1), 189-198.
61- Nathiya, S., Durga, M., & Devasena, T. (2014). Quercetin, encapsulated quercetin and its application- a review. International Journal of Pharmacy and Pharmaceutical Sciences, 6(10), 20-26.
62- Pham-Huy, L. A., He, H., & Pham-Huy, C. (2008). Free radicals, antioxidants in disease and health. International journal of biomedical science: IJBS, 4(2), 89-96.
63- Porcu, E. P., Cossu, M., Rassu, G., Giunchedi, P., Cerri, G., Pourová, J., ... & Mladěnka, P. (2018). Aqueous injection of quercetin: An approach for confirmation of its direct in vivo cardiovascular effects. International journal of pharmaceutics, 541(1-2), 224-233.
64- Ramadan, M. F., & selim Asker, M. M. (2009). Antimicrobical and antivirial impact of novel quercetin‐enriched lecithin. Journal of food biochemistry, 33(4), 557-571.
65- Rauf, A., Imran, M., Khan, I. A., ur‐Rehman, M., Gilani, S. A., Mehmood, Z., & Mubarak, M. S. (2018). Anticancer potential of quercetin: A comprehensive review. Phytotherapy research, 32(11), 2109-2130.
66- Veluri, R., Weir, T. L., Bais, H. P., Stermitz, F. R., & Vivanco, J. M. (2004). Phytotoxic and antimicrobial activities of catechin derivatives. Journal of agricultural and food chemistry, 52(5), 1077-1082.
67- Gargouri, B., Mansour, R. B., Abdallah, F. B., Elfekih, A., Lassoued, S., & Khaled, H. (2011). Protective effect of quercetin against oxidative stress caused by dimethoate in human peripheral blood lymphocytes. Lipids in health and disease, 10(1), 1-4.
68- Bu, T., Mi, Y., Zeng, W., & Zhang, C. (2011). Protective effect of quercetin on cadmium‐induced oxidative toxicity on germ cells in male mice. The Anatomical Record: Advances in Integrative Anatomy and Evolutionary Biology, 294(3), 520-526.
69- Chan, P., Cheng, J. T., Tsai, J. C., Lien, G. S., Chen, F. C., Kao, P. F., ... & Hsieh, M. H. (2002). Effect of catechin on the activity and gene expression of superoxide dismutase in cultured rat brain astrocytes. Neuroscience letters, 328(3), 281-284.
70- Kalender, Y., Yel, M., & Kalender, S. (2005). Doxorubicin hepatotoxicity and hepatic free radical metabolism in rats: the effects of vitamin E and catechin. Toxicology, 209(1), 39-45.
71- Sadowska-Krępa, E., Kłapcińska, B., Kimsa, E., & Karpińsk, R. (2008). Effects of supplemetation with red grape skin polyphenolic extract and interval swimming test on the blood antioxidant status in healthy men. Medicina Sportiva, 12(1), 1-7.

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