Fatty Acid Composition of Opuntia Ficus-Indica Seed Oil Control Angiogenic Activity in Colon Carcinoma Cell Lines

Main Article Content

Eda Becer
Hilal Kabadayı
Ali Hikmet Meriçli
Barış Kıvançlı
H. Seda Vatansever
Filiz Meriçli


Opuntia ficus-indica, colon, cancer, angiogenesis, signaling pathway


Opuntia ficus-indica belongs to the family Cactaceae that particularly rich in unsaturated fatty acids. The aim of this study was to investigate the composition and in vitro anti-angiogenic activity of spiny and thornless Opuntia ficus-indica seed  (CPS) oils. Spiny and thornless CPS oils were obtained by supercritical CO2 extraction and analyzed by GC-MS. Cell growth and cytotoxicity were measured with MTT assay with different concentrations of spiny and thornless CPS oils in Colo-320 and Colo-741 cell lines. Anti-angiogenic activity of CPS oils were investigated by immunocytochemistry using antibodies directed against to Flt-1, Flk-1, eNOS, iNOS, IL-6, PGE2, TNF-α and VEGF. Oleic acid (17.6%; 15.6%), linoleic acid (55.9%; 60.1%), palmitic acid (12.4%; 12.3%), elaidic acid (4%; 4.1%) were determined as the major compounds of spiny and thornless CPS oils, respectively. In the MTT assay, 1:16 dilution spiny CPS oil was found to be active against Colo-320 and Colo-741 cells for 48h incubation. Also, 1:8 and 1:16 dilutions of thornless CPS oil were more effective at inhibiting Colo-320 and Colo-741 for 48 h, respectively.We conclude that spiny CPS oil decreased signaling molecules which play in PGE2 mediated and VEGF-dependent angiogenesis. Interestingly, thornless CPS oil increased angiogenesis thought signaling molecules in colon cancer cells.

Abstract 394 | PDF Downloads 168


1. De Smet PA. Herbal remedies. New England Journal of Medicine. 2002;347(25):2046-56.
2. Griffith MP. The origins of an important cactus crop, Opuntia ficus‐indica (Cactaceae): new molecular evidence. American Journal of Botany. 2004;91(11):1915-21.
3. Saenz C. Processing technologies: an alternative for cactus pear (Opuntia spp.) fruits and cladodes. Journal of Arid Environments. 2000;46(3):209-25.
4. Inglese P, Basile F, Schirra M. Cactus pear fruit production. Cacti: Biology and uses. 2002:163-83.
5. Kim JH, Park SM, Ha HJ, Moon CJ, Shin TK, Kim JM, et al. Opuntia ficus-indica attenuates neuronal injury in in vitro and in vivo models of cerebral ischemia. Journal of ethnopharmacology. 2006;104(1-2):257-62.
6. Morán-Ramos S, Avila-Nava A, Tovar AR, Pedraza-Chaverri J, López-Romero P, Torres N. Opuntia ficus indica (nopal) attenuates hepatic steatosis and oxidative stress in obese Zucker (fa/fa) rats. The Journal of nutrition. 2012;142(11):1956-63.
7. Godard MP, Ewing BA, Pischel I, Ziegler A, Benedek B, Feistel B. Acute blood glucose lowering effects and long-term safety of OpunDia™ supplementation in pre-diabetic males and females. Journal of ethnopharmacology. 2010;130(3):631-4.
8. Yeddes N, Chérif JK, Jrad A, Barth D, Trabelsi-Ayadi M. Supercritical SC-CO2 and soxhlet n-hexane extract of Tunisian Opuntia ficus indica seeds and fatty acids analysis. Journal of lipids. 2012;2012.
9. Özcan MM, Al Juhaimi FY. Nutritive value and chemical composition of prickly pear seeds (Opuntia ficus indica L.) growing in Turkey. International journal of food sciences and nutrition. 2011;62(5):533-6.
10. Ciriminna R, Bongiorno D, Scurria A, Danzì C, Timpanaro G, Delisi R, et al. Sicilian Opuntia ficus‐indica seed oil: Fatty acid composition and bio‐economical aspects. European Journal of Lipid Science and Technology. 2017;119(11):1700232.
11. Becer E, Kabadayı H, Meriçli F, Meriçli AH, Kıvançlı B, Vatansever S. Apoptotic Effects of Opuntia ficus indica L. Seed Oils on Colon Adenocarcinoma Cell Lines. InMultidisciplinary Digital Publishing Institute Proceedings 2018; 2(25):1566.
12. Schmoll HJ, Van Cutsem E, Stein AE, Valentini V, Glimelius B, Haustermans K, et al. ESMO Consensus Guidelines for management of patients with colon and rectal cancer. a personalized approach to clinical decision making. Annals of oncology. 2012;23(10):2479-516.
13. Ebos JM, Lee CR, Bogdanovic E, Alami J, Van Slyke P, Francia G, et al. Vascular endothelial growth factor–mediated decrease in plasma soluble vascular endothelial growth factor receptor-2 levels as a surrogate biomarker for tumor growth. Cancer research. 2008;68(2):521-9.
14. Takahashi T, Ueno H, Shibuya M. VEGF activates protein kinase C-dependent, but Ras-independent Raf-MEK-MAP kinase pathway for DNA synthesis in primary endothelial cells. Oncogene. 1999;18(13):2221-30.
15. Ferrara N, Gerber HP, LeCouter J. The biology of VEGF and its receptors. Nature medicine. 2003;9(6):669-76.
16. Pohl M, Werner N, Munding J, Tannapfel A, Graeven U, Nickenig G, et al. Biomarkers of anti-angiogenic therapy in metastatic colorectal cancer (mCRC): original data and review of the literature. Zeitschrift für Gastroenterologie. 2011;49(10):1398-406.
17. Marrone C, Poletto M, Reverchon E, Stassi A. Almond oil extraction by supercritical CO2: experiments and modelling. Chemical Engineering Science. 1998;53(21):3711-8.
18. Gören AC, Kiliç T, Dirmenci T, Bilsel G. Chemotaxonomic evaluation of Turkish species of Salvia: Fatty acid compositions of seed oils. Biochemical Systematics and Ecology. 2006;34(2):160-4.
19. El Mannoubi I, Barrek S, Skanji T, Casabianca H, Zarrouk H. Characterization of Opuntia ficus indica seed oil from Tunisia. Chemistry of Natural Compounds. 2009;45(5):616.
20. El-Mostafa K, El Kharrassi Y, Badreddine A, Andreoletti P, Vamecq J, El Kebbaj M, et al. Nopal cactus (Opuntia ficus-indica) as a source of bioactive compounds for nutrition, health and disease. Molecules. 2014;19(9):14879-901.
21. Berraaouan A, Abderrahim Z, Hassane M, Abdelkhaleq L, Mohammed A, Mohamed B. Evaluation of protective effect of cactus pear seed oil (Opuntia ficus-indica L. MILL.) against alloxan-induced diabetes in mice. Asian Pacific journal of tropical medicine. 2015;8(7):532-7.
22. Chougui N, Tamendjari A, Hamidj W, Hallal S, Barras A, Richard T, et al. Oil composition and characterisation of phenolic compounds of Opuntia ficus-indica seeds. Food chemistry. 2013;139(1-4):796-803.
23. Coşkuner YN, Tekin A. Monitoring of seed composition of prickly pear (Opuntia ficus‐indica L) fruits during maturation period. Journal of the Science of Food and Agriculture. 2003;83(8):846-9.
24. Ellis LM. A targeted approach for antiangiogenic therapy of metastatic human colon cancer. The American surgeon. 2003;69(1):3.
25. Lesslie DP, Summy JM, Parikh NU, Fan F, Trevino JG, Sawyer TK, et al. Vascular endothelial growth factor receptor-1 mediates migration of human colorectal carcinoma cells by activation of Src family kinases. British journal of cancer. 2006;94(11):1710-7.
26. Wang D, Wang H, Shi Q, Katkuri S, Walhi W, Desvergne B, et al. Prostaglandin E2 promotes colorectal adenoma growth via transactivation of the nuclear peroxisome proliferator-activated receptor δ. Cancer cell. 2004;6(3):285-95.
27. Xu L, Croix BS. Improving VEGF-targeted therapies through inhibition of COX-2/PGE2 signaling. Molecular & cellular oncology. 2014;1(4):e969154.
28. Balkwill F. TNF-α in promotion and progression of cancer. Cancer and Metastasis Reviews. 2006;25(3):409.
29. Hussain SP, Trivers GE, Hofseth LJ, He P, Shaikh I, Mechanic LE, et al. Nitric oxide, a mediator of inflammation, suppresses tumorigenesis. Cancer research. 2004;64(19):6849-53.
30. Campelo AP, Campelo MW, Brito GA, Jamacaru FV, Leitão RF, Vasconcelos PR. Oil mixes omega 9, 6 and 3, enriched with seaweed, promoted reduction of thermal burned modulating NF-kB and Ki-67. Acta cirurgica brasileira. 2015;30(6):430-8.

Most read articles by the same author(s)